Nundaeng Supakorn, Suwannarach Nakarin, Limtong Savitree, Khuna Surapong, Kumla Jaturong, Lumyong Saisamorn
Master of Science Program in Applied Microbiology (International Program), Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
J Fungi (Basel). 2021 Nov 11;7(11):957. doi: 10.3390/jof7110957.
Ascomycetous yeast species in the genus (Saccharomycetales, Wickerhamomycetaceae) are isolated from various habitats and distributed throughout the world. Prior to this study, 35 species had been validly published and accepted into this genus. Beneficially, species have been used in a number of biotechnologically applications of environment, food, beverage industries, biofuel, medicine and agriculture. However, in some studies, species have been identified as an opportunistic human pathogen. Through an overview of diversity, taxonomy and recently published literature, we have updated a brief review of . Moreover, two new species were isolated from the soil samples of Assam tea ( var. ) that were collected from plantations in northern Thailand. Herein, we have identified these species as . and . . The identification of these species was based on phenotypic (morphological, biochemical and physiological characteristics) and molecular analyses. Phylogenetic analyses of a combination of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit (LSU) of ribosomal DNA genes support that . and . are distinct from other species within the genus . A full description, illustrations and a phylogenetic tree showing the position of both new species have been provided. Accordingly, a new combination species, . has been proposed based on the phylogenetic results. A new key for species identification is provided.
属（酵母目，威克汉姆酵母科）的子囊菌酵母物种从各种栖息地分离出来，分布于世界各地。在本研究之前，该属已有35个有效发表并被接受的物种。有益的是，该属物种已被用于环境、食品、饮料行业、生物燃料、医药和农业等许多生物技术应用中。然而，在一些研究中，该属物种已被鉴定为机会性人类病原体。通过对多样性、分类学和最近发表的文献进行综述，我们更新了对该属的简要回顾。此外，从泰国北部种植园采集的阿萨姆茶（变种）土壤样本中分离出两个新的该属物种。在此，我们将这些物种鉴定为。和。。这些物种的鉴定基于表型（形态、生化和生理特征）和分子分析。核糖体DNA基因大亚基（LSU）的内部转录间隔区（ITS）和D1/D2结构域组合的系统发育分析支持。和。与该属内的其他物种不同。提供了完整的描述、插图以及显示两个新物种位置的系统发育树。因此，根据系统发育结果提出了一个新的组合物种，。。提供了一个新的物种鉴定检索表。
