Allenmark S, Chelminska-Bertilsson M, Thompson R A
Laboratory of Microbiological Chemistry, University of Gothenburg, Sweden.
Anal Biochem. 1990 Mar;185(2):279-85. doi: 10.1016/0003-2697(90)90293-i.
Phase-transfer-catalyzed esterification of various carboxylic acids has been carried out on a microanalytical scale with a new reagent, N-(9-acridinyl)-bromoacetamide, in aqueous/organic two-phase systems. The procedure gives esters showing very high fluorescence intensity in acid solution. The large Stokes' shift (greater than 120 nm) means that a low background can be achieved even when using a large emission bandwidth. These derivatives show very good chromatographic behavior on reversed-phase liquid chromatographic columns with the use of aqueous acetonitrile, containing 0.2% of phosphoric acid, as the mobile phase. Detection limits (S/N = 2) as low as 10 fmol can be obtained under optimal conditions.
在水/有机两相体系中，使用新型试剂N-(9-吖啶基)-溴乙酰胺，在微量分析规模上进行了各种羧酸的相转移催化酯化反应。该方法得到的酯在酸性溶液中显示出非常高的荧光强度。大的斯托克斯位移（大于120 nm）意味着即使使用大的发射带宽也能实现低背景。这些衍生物在使用含0.2%磷酸的乙腈水溶液作为流动相的反相液相色谱柱上表现出非常好的色谱行为。在最佳条件下可获得低至10 fmol的检测限（S/N = 2）。
