Institut für Organische Chemie, Universität Regensburg, D-93040 Regensburg, Germany.
J Am Chem Soc. 2013 Feb 27;135(8):2967-70. doi: 10.1021/ja4001568. Epub 2013 Feb 14.
Applying molecular imprinting techniques to the surface of functionalized unilammelar fluid vesicles allows the preparation of specific and high-affinity luminescent chemosensors. We have photopolymerized diacetylene containing vesicles in the presence of small peptides as templates yielding imprinted polydiacetylene (PDA) patches in the membrane. They serve as multivalent receptor sites with significantly increased rebinding affinity for the template. All binding sites are surface exposed and accessible for analyte binding. The presence of analytes is signaled with high sensitivity by emission intensity changes of amphiphilic carboxyfluorescein, which is coembedded into the fluid DOPC membrane. The merger of PDA imprinting with dynamic functionalized vesicles overcomes some of the current limitations of molecular imprinting in chemosensor design and may be applied to many different target analytes.
将分子印迹技术应用于功能化的单分子层囊泡的表面，可以制备出具有特异性和高亲和力的发光化学传感器。我们在存在小肽作为模板的情况下，光聚合了含有二乙炔的囊泡，在膜中得到了印迹的聚二乙炔（PDA）补丁。它们作为多价受体位点，对模板的再结合亲和力显著增加。所有的结合位点都暴露在表面，可用于分析物的结合。两亲性羧基荧光素的发射强度变化可以高灵敏度地指示分析物的存在，该荧光素共嵌入到流体 DOPC 膜中。PDA 印迹与动态功能化囊泡的结合克服了化学传感器设计中分子印迹的一些当前限制，并且可以应用于许多不同的目标分析物。
