Institute of Molecular Cardiology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Arch Physiol Biochem. 2013 May;119(2):39-51. doi: 10.3109/13813455.2012.755547. Epub 2013 Feb 11.
CD73 converts extracellular AMP to adenosine which is well known to inhibit lipolysis. It is unknown, however, whether adenosine formed directly by CD73 is functionally relevant in this process.
We therefore explored the effect of CD73-derived adenosine on body fat of aged mice.
In lean mice, extracellular adenosine formation by adipocytes is dependent on CD73. High fat diet down-regulates the expression of CD73 in wildtype mice similar to ob/ob mice. Transgenic mice chronically lacking CD73 (CD73(-/-)) gain significantly less body weight and show decreased superficial white fat content as well as increased serum free fatty acids and triglycerides. In addition, intramyocellular lipid levels are significantly increased. This phenotype is accompanied by an increase in blood glucose and serum insulin levels although insulin secretion and the level of insulin degrading enzyme are unaltered. Additionally, insulin-induced Akt phosphorylation is reduced in skeletal muscle of CD73(-/-) mice.
CD73-derived adenosine is functionally involved in body fat homeostasis.
CD73 将细胞外 AMP 转化为腺苷，众所周知，腺苷能抑制脂肪分解。然而，尚不清楚 CD73 直接形成的腺苷在此过程中是否具有功能相关性。
因此，我们探讨了 CD73 衍生的腺苷对老年小鼠体脂肪的影响。
在瘦小鼠中，脂肪细胞的细胞外腺苷形成依赖于 CD73。高脂肪饮食下调野生型小鼠和 ob/ob 小鼠中 CD73 的表达。慢性缺乏 CD73 的转基因小鼠（CD73(-/-)）体重显著增加，浅层白色脂肪含量减少，血清游离脂肪酸和甘油三酯增加。此外，肌内脂质水平显著增加。这种表型伴随着血糖和血清胰岛素水平的升高，尽管胰岛素分泌和胰岛素降解酶的水平没有改变。此外，CD73(-/-)小鼠骨骼肌中胰岛素诱导的 Akt 磷酸化减少。
CD73 衍生的腺苷在体脂肪稳态中具有功能作用。
