Topping M D, Brighton W D, Stokell M, Patterson J M
J Immunol Methods. 1978;19(1):61-7. doi: 10.1016/0022-1759(78)90008-x.
The technique of isoelectric focussing in polyacrylamide gel has been developed to a preparative mode. Using the improved technique cocksfoot (Dactylis glomerata) pollen has been split into many components, and the allergenic reactions of the preparations have been studied by passive transfer in animal skin and by RAST.
聚丙烯酰胺凝胶等电聚焦技术已发展到制备模式。利用改进后的技术,已将鸭茅(Dactylis glomerata)花粉分离成多个组分,并通过动物皮肤被动转移和放射变应原吸附试验(RAST)研究了这些制剂的过敏反应。