Seagroatt V, Ford A
Clin Allergy. 1986 May;16(3):213-20. doi: 10.1111/j.1365-2222.1986.tb00768.x.
The suitability and reproducibility of the inhibition mode of the radioallergosorbent test (RAST) as a method for the assay of allergen extracts was examined in a collaborative study involving seven laboratories. Participants assayed five coded extracts of cocksfoot grass (Dactylis glomerata) pollen, including three batches from one manufacturer, both by a common method and by their local methods of RAST inhibition. For the versions of RAST inhibition employed in this study the estimates of relative potencies of the extracts were in agreement. Further, when laboratories used their local methods, the inter-laboratory variation in potency estimates was similar to that found when laboratories used the common method. It was concluded that RAST inhibition has a useful role in the assessment of the potencies of allergen extracts, and of the consistency of serial batches of extracts from individual manufacturers.
在一项涉及七个实验室的合作研究中，对放射变应原吸附试验（RAST）抑制模式作为过敏原提取物测定方法的适用性和可重复性进行了检验。参与者采用通用方法和各自实验室的RAST抑制法，对五种编码的鸭茅（Dactylis glomerata）花粉提取物进行了测定，其中包括来自同一制造商的三批产品。对于本研究中采用的RAST抑制法版本，提取物相对效价的估计值是一致的。此外，当各实验室使用各自的方法时，效价估计的实验室间差异与使用通用方法时相似。得出的结论是，RAST抑制法在评估过敏原提取物的效价以及单个制造商连续批次提取物的一致性方面具有重要作用。
