Karolinska Institutet, Department of Clinical Neuroscience, Center for Psychiatric Research, Stockholm, Sweden.
J Chromatogr A. 2013 Mar 15;1281:54-9. doi: 10.1016/j.chroma.2013.01.071. Epub 2013 Jan 23.
A mixed micellar liquid chromatographic (LC) method, the mobile phase consisting of anionic and non-ionic surfactants, has been developed for the high-speed direct radiometabolite analysis of positron emission tomography (PET) radioligands in plasma. The addition of Triton X-100 on an anionic surfactant sodium dodecyl sulphate (SDS) mobile phase improved elution strength and peak efficiency for many PET radioligands. Several radioligands could be easily separated from their radioactive metabolites with short run time of only 4 min using a "pure" (without organic solvent) mixed micellar mobile phase and semi-preparative monolithic C(18)-bonded silica column by simple isocratic elution without any treatment of plasma. Moreover, the use of "hybrid" mixed micellar mobile phase containing anionic, non-ionic surfactants and organic solvent was effective to further enhance peak efficiency and elute highly retained hydrophobic PET radioligands. These characteristics enabled significant shorting the radiometabolite analysis procedure of PET radioligands and simplifying the experimental setup.
建立了一种混合胶束液相色谱（LC）法，流动相由阴离子和非离子表面活性剂组成，用于快速直接放射代谢产物分析正电子发射断层扫描（PET）放射性配体在血浆中。在阴离子表面活性剂十二烷基硫酸钠（SDS）流动相中加入 Triton X-100 可提高许多 PET 放射性配体的洗脱强度和峰效。使用“纯”（不含有机溶剂）混合胶束流动相和半制备整体式 C(18)-键合硅胶柱，通过简单的等度洗脱，无需对血浆进行任何处理，仅用 4 分钟的短运行时间即可轻松将几种放射性配体与其放射性代谢物分离。此外，使用含有阴离子、非离子表面活性剂和有机溶剂的“混合”混合胶束流动相可有效进一步提高峰效率并洗脱高度保留的疏水性 PET 放射性配体。这些特点使 PET 放射性配体的放射代谢产物分析程序显著缩短，实验装置简化。
