Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, Lanzhou 730000, China.
Carbohydr Polym. 2013 Feb 15;92(2):1124-32. doi: 10.1016/j.carbpol.2012.10.022. Epub 2012 Oct 17.
Core-shell structured multifunctional nanocarriers (NCs) of ZnO quantum dots-conjugated gold nanoparticles (Au NPs) as core and amphiphilic hyperbranched block copolymer as shell were synthesized for targeted anticancer drug delivery. The amphiphilic hyperbranched block copolymer contained poly(l-lactide) (PLA) inner arm and folate (FA)-conjugated a sulfated polysaccharide from Gynostemma pentaphyllum Makino (GPPS-FA) outer arm. The structure and properties of core-shell structured multifunctional nanocarriers were characterized and determined by UV-visible spectra, FT-IR spectra, X-ray diffraction (XRD), fluorescence spectroscopy and TEM analyses. The release results indicated that camptothecin (CPT) release from NCs at pH 7.4 was much greater than that at pH 5.3. The cytotoxicity studies showed that both the blank NCs and the CPT-loaded NCs provided high anticancer activity against Hela cells. Furthermore, nanocarriers gained specificity to target model cancer cells in this study due to the enhanced cell uptake mediated by FA moiety. The results indicated that the NCs not only had great potential as tumor-targeted drug delivery nanocarrier, but also had an assistant role in the treatment of cancer.
基于 ZnO 量子点-金纳米粒子（Au NPs）核壳结构的多功能纳米载体（NCs）被合成出来，用于靶向抗癌药物传递。该两亲超支化嵌段共聚物的内核为聚（L-乳酸）（PLA），外壳为叶酸（FA）修饰的绞股蓝多糖（GPPS-FA）。通过紫外-可见光谱、傅里叶变换红外光谱、X 射线衍射（XRD）、荧光光谱和透射电子显微镜分析对核壳结构多功能纳米载体的结构和性能进行了表征和确定。释放结果表明，在 pH7.4 时，CPT 从 NCs 的释放量明显大于在 pH5.3 时的释放量。细胞毒性研究表明，空白 NCs 和载 CPT 的 NCs 对 Hela 细胞均具有较高的抗癌活性。此外，由于 FA 部分介导的细胞摄取增强，本研究中的纳米载体对靶标模型癌细胞具有特异性。结果表明，NCs 不仅具有作为肿瘤靶向药物传递纳米载体的巨大潜力，而且在癌症治疗中也具有辅助作用。
