School of Biology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
Carbohydr Polym. 2013 Feb 15;92(2):1211-7. doi: 10.1016/j.carbpol.2012.10.037. Epub 2012 Oct 23.
The protective effect of an alpha-glucan (designated here as MT-α-glucan) from fruit body of maitake (Grifola frondosa) on NIT-1 pancreatic β-cells damaged by streptozotocin (STZ) in vitro was investigated. The cell viability, insulin secretion, the activity of superoxide dismutase (SOD), glutathione peroxidase (GSHpx) and the content of reduced glutathione (GSH) increased significantly when the cells were incubated with MT-α-glucan (400, 200 μg ml(-1)). The content of malondialdehyde (MDA), nitric oxide (NO) production, and the activity of NO synthase (NOS), inducible NOS (iNOS) decreased significantly when the cells were incubated with MT-α-glucan. The destructive changes of NIT-1 cells ameliorated when incubated with MT-α-glucan under microscopic observation. These data suggested that MT-α-glucan had obvious protective effect on NIT-1 pancreatic β-cells damaged by STZ, which might be related to its effects of decreasing levels of β-cell-destroying factors such as oxidative stress and NO synthesis.
从药用蘑菇（灰树花）的子实体中提取的一种α-葡聚糖（此处称为 MT-α-葡聚糖）对体外链脲佐菌素（STZ）损伤的 NIT-1 胰岛β细胞的保护作用进行了研究。当细胞与 MT-α-葡聚糖（400、200μg/ml）孵育时，细胞活力、胰岛素分泌、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSHpx）活性和还原型谷胱甘肽（GSH）含量显著增加。当细胞与 MT-α-葡聚糖孵育时，丙二醛（MDA）含量、一氧化氮（NO）生成、一氧化氮合酶（NOS）和诱导型NOS（iNOS）活性显著降低。在显微镜下观察到，当用 MT-α-葡聚糖孵育时，NIT-1 细胞的破坏变化得到改善。这些数据表明，MT-α-葡聚糖对 STZ 损伤的 NIT-1 胰岛β细胞具有明显的保护作用，这可能与其降低β细胞破坏因子（如氧化应激和 NO 合成）水平的作用有关。
