Hong Lei, Xun Ma, Wutong Wu
School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.
J Pharm Pharmacol. 2007 Apr;59(4):575-82. doi: 10.1211/jpp.59.4.0013.
We have evaluated the anti-diabetic effect of a alpha-glucan (MT-alpha-glucan) from the fruit body of maitake mushrooms (Grifola frondosa) on KK-Ay mice (a kind of genetical type 2 diabetes animal model). The effects of MT-alpha-glucan (450 or 150 mg kg (-1)) on diabetic mice were investigated by observing the changes in body weight, the level of fasting plasma glucose, glycosylated serum protein (GSP), hepatic glycogen, serum insulin, triglycerides, cholesterol, free fatty acid, liver superoxide dismutase (SOD), glutathione peroxidase (GSHpx), reduced glutathione (GSH) and malondialdehyde (MDA). Moreover, the binding capacity of insulin receptors on liver crude plasma membranes was assayed and histopathological changes in the pancreas were observed. Treatment with MT-alpha-glucan significantly decreased the body weight, level of fasting plasma glucose, GSP, serum insulin, triglycerides, cholesterol, free fatty acid and MDA content in livers. Treatment with MT-alpha-glucan significantly increased the content of hepatic glycogen, GSH and the activity of SOD and GSHpx. Moreover, the insulin binding capacity to liver crude plasma membranes increased and histopathological changes in the pancreas were ameliorated in the treatment group. These data suggest that MT-alpha-glucan has an anti-diabetic effect on KK-Ay mice, which might be related to its effect on insulin receptors (i.e., increasing insulin sensitivity and ameliorating insulin resistance of peripheral target tissues).
我们评估了从舞茸（灰树花）子实体中提取的一种α-葡聚糖（MT-α-葡聚糖）对KK-Ay小鼠（一种遗传性2型糖尿病动物模型）的抗糖尿病作用。通过观察体重、空腹血糖水平、糖化血清蛋白（GSP）、肝糖原、血清胰岛素、甘油三酯、胆固醇、游离脂肪酸、肝脏超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSHpx）、还原型谷胱甘肽（GSH）和丙二醛（MDA）的变化，研究了MT-α-葡聚糖（450或150 mg kg(-1)）对糖尿病小鼠的影响。此外，还检测了肝粗质膜上胰岛素受体的结合能力，并观察了胰腺的组织病理学变化。MT-α-葡聚糖治疗显著降低了体重、空腹血糖水平、GSP、血清胰岛素、甘油三酯、胆固醇、游离脂肪酸和肝脏中的MDA含量。MT-α-葡聚糖治疗显著增加了肝糖原、GSH的含量以及SOD和GSHpx的活性。此外，治疗组肝粗质膜上的胰岛素结合能力增加，胰腺的组织病理学变化得到改善。这些数据表明，MT-α-葡聚糖对KK-Ay小鼠具有抗糖尿病作用，这可能与其对胰岛素受体的作用有关（即增加胰岛素敏感性，改善外周靶组织的胰岛素抵抗）。
