College of Environmental Science and Engineering, Anhui Normal University, Wuhu 241000, China.
Carbohydr Polym. 2013 Feb 15;92(2):1608-11. doi: 10.1016/j.carbpol.2012.09.052. Epub 2012 Oct 4.
Difructosan anhydrides III (DFA III) preparation was usually obtained by inulin hydrolysis with inulin fructotransferase (IFTase). The fructofuranosidic linkages of inulin were the same as fructooligosaccharides (FOS), which was synthesized by sucrose with fructosyltransferase (FTase). FOS was mainly composed of 1-kestose (GF(2)), nystose (GF(3)) and fructofuranosylnystose (GF(4)), and nystose was observed to be the smallest substrate for IFTase to synthesize DFA III. So sucrose, much cheaper than inulin, was considered to produce DFA III by coupled FTase and IFTase reaction. DFA III yield was obtained about 100mg/g (DFA III weight/sucrose weight) through this method. The results demonstrated the high potential of the coupled enzyme reaction as a novel DFA III producing method.
菊粉三酐（DFA III）的制备通常通过菊粉果糖基转移酶（IFTase）水解菊粉获得。菊粉的呋喃果糖苷键与低聚果糖（FOS）相同，FOS 是由蔗糖与果糖基转移酶（FTase）合成的。FOS 主要由 1-蔗果三糖（GF（2））、蔗果四糖（GF（3））和蔗果三糖（GF（4））组成，研究表明，nystose 是 IFTase 合成 DFA III 的最小底物。因此，与菊粉相比，蔗糖价格便宜得多，被认为可以通过偶联 FTase 和 IFTase 反应来生产 DFA III。通过这种方法，DFA III 的产率约为 100mg/g（DFA III 重量/蔗糖重量）。结果表明，偶联酶反应具有作为一种新型 DFA III 生产方法的巨大潜力。
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