State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
J Biosci Bioeng. 2012 Jan;113(1):55-7. doi: 10.1016/j.jbiosc.2011.08.026. Epub 2011 Oct 20.
An ultrafiltration membrane bioreactor was used for the production of DFA III from enzymatic conversion of inulin. Compared with the traditional batch reactor, the productivity and purity of DFA III could be markedly enhanced and product inhibition was removed and IFTase could be continuously used for six runs in the UF membrane bioreactor. When the substrate concentration was 100 g/L, the concentration of DFA III was about 78.4 g/L, while the productivity and purity of DFA III could attain about 2385 and 92%, respectively.
采用超滤膜生物反应器从菊粉酶转化生产 DFA III。与传统的分批式反应器相比，超滤膜生物反应器可明显提高 DFA III 的产率和纯度，去除产物抑制，并使 IFTase 能够在超滤膜生物反应器中连续使用六次。当底物浓度为 100 g/L 时，DFA III 的浓度约为 78.4 g/L，而 DFA III 的产率和纯度分别可达 2385 和 92%左右。
