Ueda M, Sashida R, Morimoto Y, Ohkishi H
Mitsubishi Kasei Corporation, Research Center, Yokohama, Japan.
Biosci Biotechnol Biochem. 1994 Mar;58(3):574-5. doi: 10.1271/bbb.58.574.
An extracellular enzyme that produces di-D-fructofuranose-2',1;2,1' dianhydride from inulin was purified from the culture broth of Arthrobacter sp. MCI2493. The molecular weight of the enzyme was 40,000 by gel filtration and SDS polyacrylamide gel electrophoresis. The enzyme had maximum activity at pH 6.0 and 50 degrees C. Using this purified enzyme, 100 g/liter inulin was converted into 60 g/liter of DFA I, nystose, and 1-F-fructofuranosyl-nystose after incubation for 30 h.
从节杆菌属菌株MCI2493的培养液中纯化出一种能将菊粉转化为二 - D - 呋喃果糖 - 2',1;2,1' 二酐的胞外酶。通过凝胶过滤和SDS聚丙烯酰胺凝胶电泳测定，该酶的分子量为40,000。该酶在pH 6.0和50℃时具有最大活性。使用这种纯化的酶，将100克/升的菊粉孵育30小时后，可转化为60克/升的DFA I、蔗果三糖和1 - F - 呋喃果糖基 - 蔗果三糖。
