Institute of Chemistry of Komi Science Centre of the Urals Branch of the Russian Academy of Sciences, Syktyvkar 167982, Russia.
Institute of Chemistry of Komi Science Centre of the Urals Branch of the Russian Academy of Sciences, Syktyvkar 167982, Russia.
Int J Biol Macromol. 2019 Feb 1;122:29-36. doi: 10.1016/j.ijbiomac.2018.10.146. Epub 2018 Oct 22.
The polysaccharide PG was isolated from peels and membranes of fruits of pomegranate P. granatum by hot water extraction. The methods of ion exchange chromatography, partial acid hydrolysis, enzymatic hydrolysis and NMR spectroscopy were employed to establish the major elements of its structure. It was shown that this polysaccharide contained polymers with different structures, the main components among which were pectic polysaccharides, represented mainly by highly methyl-esterified and lowly acetylated 1,4-α-d-galactopyranosyluronan and a minor amount of partially 2-O- and/or 3-O-acetylated RG-I. The side carbohydrate chains of the branched region of RG-I were mainly represented by terminal, 5-O-, 3-O-, 2,5-di-O-, 3,5-di-O- and 2,3,5-tri-O-substituted α-l-Araf residues indicating the presence of branched 1,5-α-l-arabinan and minor regions of 1,4-β-d-galactan or arabinogalactan type I. The degree of methylation of the isolated pectins varied depending on the method of treatment. As a result, peels and membranes of pomegranate fruits can be recommended as a source of highly and lowly methyl-esterified pectic polysaccharides.
从石榴（Punica granatum）果皮和膜中用热水提取分离得到多糖 PG。采用离子交换色谱、部分酸水解、酶水解和 NMR 光谱法确定其结构的主要元素。结果表明，该多糖含有不同结构的聚合物，主要成分是果胶多糖，主要由高度甲酯化和低度乙酰化的 1,4-α-d-半乳糖吡喃糖醛酸和少量部分 2-O-和/或 3-O-乙酰化 RG-I 组成。RG-I 支链区的侧链碳水化合物主要由末端、5-O-、3-O-、2,5-二-O-、3,5-二-O-和 2,3,5-三-O-取代的α-l-阿拉伯呋喃糖残基表示，存在支链 1,5-α-l-阿拉伯聚糖和较小区域的 1,4-β-d-半乳糖或阿拉伯半乳聚糖 I 型。分离出的果胶的甲酯化程度取决于处理方法。因此，石榴果皮和膜可作为高度和低度甲酯化果胶多糖的来源。
