School of Environmental and Biological Engineering, Liaoning Shihua University, Fushun 113001, PR China.
Carbohydr Polym. 2013 Feb 15;92(2):2187-92. doi: 10.1016/j.carbpol.2012.12.011. Epub 2012 Dec 12.
Two novel polysaccharide fractions, GRMP1 and GRMP2, were isolated from the mycelium of Gomphidius rutilus through submerged fermentation. GRMP1 and GRMP2 had similar average molecular weights (35 and 31 kDa, respectively), and were composed of glucose and xylose at molar ratios of 0.46:1 and 0.63:1, respectively. In vitro antioxidant tests showed that GRMP1 and GRMP2 partly scavenged superoxide radical but almost had no scavenging effect on 1,1-diphenyl-2-picrylhydrazyl free radical. The effect of GRMP1 on hydroxyl radicals was stronger than that of GRMP2. Both GRMP1 and GRMP2 had relatively low reducing power and significant lymphocyte proliferation activity. In the presence of concanavalin A or lipopolysaccharide as mitogens for lymphocytes, the lymphocyte proliferation activity increased for GRMP1 but not for GRMP2 within the test dosage range.
两种新型多糖成分，GRMP1 和 GRMP2，是通过液体深层发酵从红缘拟层孔菌菌丝体中分离得到的。GRMP1 和 GRMP2 的平均分子量相近（分别为 35 和 31 kDa），均由葡萄糖和木糖组成，摩尔比分别为 0.46:1 和 0.63:1。体外抗氧化试验表明，GRMP1 和 GRMP2 部分清除超氧自由基，但对 1,1-二苯基-2-苦基肼自由基几乎没有清除作用。GRMP1 对羟自由基的作用强于 GRMP2。GRMP1 和 GRMP2 的还原能力均较低，但具有显著的淋巴细胞增殖活性。在有刀豆蛋白 A 或脂多糖作为淋巴细胞有丝分裂原的情况下，在试验剂量范围内，GRMP1 对淋巴细胞的增殖活性增加，但 GRMP2 没有。
Zotero 插件