Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing, P.R. China.
College of Life Science, Shandong Agricultural University, Taian, 271018, P.R. China.
Sci Rep. 2018 May 15;8(1):7574. doi: 10.1038/s41598-018-25830-w.
The present work was designed to evaluate the antioxidation and hepatoprotective effects of Auricularia cornea var. Li. polysaccharides (APS) and enzymatic-extractable APS (EAPS) on the acute alcohol-induced alcoholic liver diseases (ALD). The in vitro antioxidant activities demonstrated that both APS and EAPS had strong reducing power and potential effects on scavenging reactive oxygen species. The in vivo mice experiments showed that the pretreatment with APS or EAPS showed potential hepatoprotective effects on the ALD possibly by increasing the antioxidant activities, reducing the lipid peroxidation, improving the alcohol metabolism, inhibiting the expression levels of inflammatory mediators and preventing the alcohol-induced histopathological alterations. In addition, the fourier-transform infrared (FT-IR), H and C nuclear magnetic resonance spectroscopy (NMR) and gas chromatography (GC) had been analyzed to obtained the primarily characteristics. The results indicated that abundant xylose and glucose contents probably had potential effects on possessing the bioactivities. The findings suggested that the A. cornea var. Li. might be considered as promising natural resource on exploring clinical drugs for the prevention and treatment with ALD and its complications.
本研究旨在评估银耳多糖（APS）和酶提取银耳多糖（EAPS）对急性酒精诱导的酒精性肝病（ALD）的抗氧化和保肝作用。体外抗氧化活性研究表明，APS 和 EAPS 均具有较强的还原能力和清除活性氧的潜力。体内小鼠实验表明，APS 或 EAPS 的预处理对 ALD 具有潜在的保肝作用，可能是通过增加抗氧化活性、降低脂质过氧化、改善酒精代谢、抑制炎症介质的表达水平和防止酒精引起的组织病理学改变来实现的。此外，还通过傅里叶变换红外（FT-IR）、氢和碳核磁共振波谱（NMR）和气相色谱（GC）进行了分析以获得主要特征。结果表明，丰富的木糖和葡萄糖含量可能对具有生物活性具有潜在作用。这些发现表明，银耳可能被认为是一种有前途的天然资源，可用于开发预防和治疗 ALD 及其并发症的临床药物。
