School of Environmental and Biological Engineering, Liaoning Shihua University, Fushun 113001, China.
Carbohydr Polym. 2013 Apr 15;94(1):479-86. doi: 10.1016/j.carbpol.2013.01.034. Epub 2013 Jan 23.
Response surface methodology was used to optimize the extraction of crude polysaccharides from the fruiting bodies of Gomphidius rutilus. A central composite design was adopted to determine the combination of factors (extraction time, extraction temperature, extraction frequency, and ratio of water to raw material) that resulted in the maximum crude polysaccharide production. Results showed that the optimum extraction conditions were as follows: extraction temperature, 95°C; ratio of water to raw material, 16; extraction time, 2.5 h; and extraction frequency, 4. Under these conditions, the experimental yield of crude polysaccharides was 8.02±0.15%, which well agreed with the predicted yield. Evaluation of the antioxidant activity in vitro suggested that the crude polysaccharides had high scavenging activity for superoxide anion, hydroxyl, and 1,1-diphenyl-2-picrylhydrazyl radicals. The crude polysaccharides also showed strong reducing power. Thus, they can be used as natural antioxidants in functional foods or medicine.
响应面法被用于优化从蝉花的子实体中提取粗多糖。采用中心复合设计确定了导致最大粗多糖产量的因素（提取时间、提取温度、提取频率和水与原料的比例）的组合。结果表明，最佳提取条件为：提取温度 95°C，水与原料的比例为 16，提取时间为 2.5 h，提取频率为 4。在这些条件下，粗多糖的实验产率为 8.02±0.15%，与预测产率吻合良好。体外抗氧化活性评价表明，粗多糖对超氧阴离子、羟基和 1,1-二苯基-2-苦基肼自由基具有很高的清除活性。粗多糖还表现出很强的还原能力。因此，它们可以用作功能性食品或药物中的天然抗氧化剂。
