Department of Chemical Technology, University of Johannesburg, P.O. Box 17011, Doornfontien 2028, Johannesburg, South Africa.
Carbohydr Polym. 2013 Feb 15;92(2):2201-5. doi: 10.1016/j.carbpol.2012.11.102. Epub 2012 Dec 10.
Enantiomeric resolution of α-amino acids into L-amino acid and D-amino acid via column chromatography using chiral stationary phase was performed. For this purpose, a dynamic chiral stationary phase prepared by grafting of methylmethacrylate onto xanthan gum (XG) was successfully employed in resolving various α-amino acids racemates. The peculiarities of the chromatographic behaviour of xanthan gum-graft-poly(methylmethacrylate)-amino acid interaction and the mechanism of their retention in column are discussed. The enantioselective properties of the xanthan gum-graft-poly(methylmethacrylate) in the separation of enantiomers of α-amino acids were studied using acidic solution of alanine, leucine, valine and tryptophan. The procedure is characterized by simplicity, efficiency and relatively low cost to analyze enantiomers of some amino acids.
通过使用手性固定相进行柱层析，将 α-氨基酸对映体拆分成为 L-氨基酸和 D-氨基酸。为此，成功地使用通过将甲基丙烯酸甲酯接枝到黄原胶 (XG) 上制备的动态手性固定相来拆分各种 α-氨基酸外消旋体。讨论了黄原胶-接枝-聚(甲基丙烯酸甲酯)-氨基酸相互作用的色谱行为的特点及其在柱中保留的机制。使用丙氨酸、亮氨酸、缬氨酸和色氨酸的酸性溶液研究了黄原胶-接枝-聚(甲基丙烯酸甲酯)在拆分 α-氨基酸对映体中的对映选择性。该方法的特点是简单、高效，并且分析一些氨基酸的对映体的成本相对较低。
