Swartz M E, Mazzeo J R, Grover E R, Brown P R
Waters Corporation, Milford, Massachusetts 01757, USA.
Anal Biochem. 1995 Oct 10;231(1):65-71. doi: 10.1006/abio.1995.1504.
A synthetic chiral surfactant was employed for the enantioselective micellar electrokinetic capillary chromatographic (MECC) separation of amino acid enantiomers derivatized with 6-aminoquinoyl-N-hydroxysuccinimidyl carbamate (AQC). The effect of surfactant concentration and buffer pH on resolution was studied, and the optimized conditions were used to evaluate the method in terms of sensitivity, reproducibility, and linearity. Resolution and alpha values for 12 ACQ-derivatized amino acids are reported. The ability to perform both achiral and chiral separations simultaneously is illustrated in a separation of a mixture of six amino acid enantiomeric pairs, all with baseline resolution. The direct reversal of enantiomer migration order, useful in quantitation and chiral identification, is also shown. Comparisons with other N-protected amino acid derivatives are made in terms of resolution and sensitivity, and the advantages of this chiral MECC technique used in conjunction with the inherent advantages of the AQC derivatizing reagent are discussed.
一种合成手性表面活性剂被用于对用6-氨基喹啉-N-羟基琥珀酰亚胺基氨基甲酸酯（AQC）衍生化的氨基酸对映体进行对映选择性胶束电动毛细管色谱（MECC）分离。研究了表面活性剂浓度和缓冲液pH对分离度的影响，并使用优化条件从灵敏度、重现性和线性方面对该方法进行评估。报告了12种AQC衍生化氨基酸的分离度和α值。在一次同时分离六个氨基酸对映体对的混合物且所有对映体对均达到基线分离度的过程中，展示了同时进行非手性和手性分离的能力。还展示了对映体迁移顺序的直接反转，这在定量分析和手性鉴定中很有用。在分离度和灵敏度方面与其他N-保护氨基酸衍生物进行了比较，并讨论了这种手性MECC技术与AQC衍生化试剂固有优势相结合的优点。
