Department of Chemistry, Division of Natural Sciences & Engineering, University of South Carolina Upstate, Spartanburg, SC 29303, USA.
Chemosphere. 2013 Jun;91(11):1643-7. doi: 10.1016/j.chemosphere.2012.12.045. Epub 2013 Feb 8.
Bisphenol A (BPA) is an environmental contaminant that has been under investigation for its detrimental effect on the aquatic ecosystem. In this study, an aquatic fungus, Heliscus lugdunensis Saccardo & Therry (live and heat-inactivated by autoclaving) was evaluated for its ability to utilize BPA as a carbon source. Both live and heat-inactivated fungal cultures were exposed to 10 mg L(-1) bisphenol A after cultivation in glucose for 3d. Extraction of BPA in the medium was carried out using PrepSep™ C-18 mini columns followed by quantification of recovered BPA by high performance liquid chromatography after sample clean-up. While heat inactivation sufficiently disrupts the ability of the fungal cells to utilize BPA, the live fungal cultures removed over 70% of BPA after 12d. Our results suggest that H. lugdunensis is capable of biodegrading BPA and utilizing it as an energy source.
双酚 A（BPA）是一种环境污染物，其对水生生态系统的有害影响一直在研究之中。在本研究中，评估了水生真菌 Heliscus lugdunensis Saccardo & Therry（通过高压灭菌进行活的和热失活）利用 BPA 作为碳源的能力。在葡萄糖中培养 3 天后，将活的和热失活的真菌培养物暴露于 10mg/L 的双酚 A 下。使用 PrepSep™ C-18 微型柱从培养基中提取 BPA，然后在样品净化后通过高效液相色谱法定量回收的 BPA。虽然热失活足以破坏真菌细胞利用 BPA 的能力，但活的真菌培养物在 12 天后去除了超过 70%的 BPA。我们的结果表明，H. lugdunensis 能够生物降解 BPA 并将其用作能源。
