Department of Biochemistry, MD (Maharshi Dayanand) University, Rohtak, India.
Anal Biochem. 2013 Jun 1;437(1):1-9. doi: 10.1016/j.ab.2013.01.030. Epub 2013 Feb 8.
An improved D-amino acid biosensor was constructed based on covalent immobilization of D-amino acid oxidase onto carboxylated mutliwalled carbon nanotube/copper nanoparticles/polyalinine hybrid film electrodeposited on gold electrode. The biosensor exhibited an optimal response within 2s at pH 8.0 and 30°C when polarized at 0.09 V. There was a linear relationship between biosensor response (μA) and D-alanine concentration ranging from 0.001 to 0.7 mM. The sensitivity of the biosensor was 54.85 μA cm(-2) mM(-1) with a lower limit of detection of 0.2 μM (signal/noise=3). The enzyme electrode was used 150 times over a period of 4 months. The biosensor measured the d-amino acid level in fruit juices.
基于共价固定化 D-氨基酸氧化酶到羧基化多壁碳纳米管/铜纳米粒子/聚多巴胺混合膜上，构建了一种改良的 D-氨基酸生物传感器，该混合膜电沉积在金电极上。当在 0.09 V 下极化时，生物传感器在 pH 8.0 和 30°C 下 2s 内达到最佳响应。当 D-丙氨酸浓度在 0.001 至 0.7 mM 范围内时，生物传感器的响应（μA）与 D-丙氨酸浓度之间存在线性关系。生物传感器的灵敏度为 54.85 μA cm(-2) mM(-1)，检测限为 0.2 μM（信号/噪声=3）。在 4 个月的时间内，酶电极使用了 150 次。该生物传感器测量了果汁中的 D-氨基酸水平。
