Li Chaofeng, Zheng Jiyang, Wu Yushuang, Wang Xiaotong, Shao Hui, Yan Dong
Shanghai Lumy Biotechnology Co., Ltd., Shanghai 201100, China.
Life (Basel). 2022 Oct 3;12(10):1537. doi: 10.3390/life12101537.
Light-driven synthetic biology refers to an autotrophic microorganisms-based research platform that remodels microbial metabolism through synthetic biology and directly converts light energy into bio-based chemicals. This technology can help achieve the goal of carbon neutrality while promoting green production. Cyanobacteria are photosynthetic microorganisms that use light and CO for growth and production. They thus possess unique advantages as "autotrophic cell factories". Various fuels and chemicals have been synthesized by cyanobacteria, indicating their important roles in research and industrial application. This review summarized the progresses and remaining challenges in light-driven cyanobacterial cell factory. The choice of chassis cells, strategies used in metabolic engineering, and the methods for high-value CO utilization will be discussed.
光驱动合成生物学是指一个基于自养微生物的研究平台，该平台通过合成生物学重塑微生物代谢，并将光能直接转化为生物基化学品。这项技术有助于实现碳中和目标，同时促进绿色生产。蓝细菌是利用光和二氧化碳进行生长和生产的光合微生物。因此，它们作为“自养细胞工厂”具有独特优势。蓝细菌已合成了各种燃料和化学品，表明它们在研究和工业应用中具有重要作用。本综述总结了光驱动蓝细菌细胞工厂的研究进展和尚存的挑战。将讨论底盘细胞的选择、代谢工程中使用的策略以及高价值二氧化碳利用方法。
