Instituto de Biotecnologia, Universidade de Caxias do Sul-UCS, Caxias do Sul, 95070-560 Rio Grande do Sul, Brazil.
Nutr Res. 2013 Feb;33(2):120-5. doi: 10.1016/j.nutres.2012.12.002. Epub 2013 Jan 17.
Oxidative damages in hepatocytes may be caused by epilepsy and/or anticonvulsant drugs. Epilepsy is one of the most common neurological disorders, characterized by recurrent seizures, which may increase the content of reactive oxygen species. Organic and conventional grape juices are rich in polyphenols, compounds with important antioxidant activity. It is hypothesized that organic and conventional purple grape juices may have protective effect against oxidative damage induced by pentylenetetrazole (PTZ) (a standard convulsant drug) in the liver and serum of Wistar rats. Animals (n = 16 in each group) received, by gavage, saline, organic grape juice or conventional grape juice (10 μL/g of body weight) for 17 days. Subsequently, half of the rats in each group received PTZ (60 mg/kg). After 30 minutes, the animals were euthanized by decapitation. Liver and blood samples were isolated to evaluate oxidative parameters (lipid and protein oxidation, nitric oxide metabolite content, antioxidant defenses, and protein sulfhydryl content). The results of this study showed that although organic juice contains higher polyphenol content than conventional juice, both juices conferred protection against lipid and protein oxidative damage and limited the increase in PTZ-induced nitric oxide metabolite content in the liver and serum. In addition, both juices inhibited the PTZ-induced reduction in enzymatic antioxidant defenses (superoxide dismutase and catalase activities) and sulfhydryl protein content in the liver and serum. In summary, both organic and conventional grape juices were able to reduce oxidative damage induced by PTZ in the liver and serum of Wistar rats.
肝细胞的氧化损伤可能是由癫痫和/或抗癫痫药物引起的。癫痫是最常见的神经系统疾病之一，其特征是反复发作，这可能会增加活性氧的含量。有机和常规葡萄汁富含多酚，这些化合物具有重要的抗氧化活性。据推测，有机和常规的紫葡萄汁可能对戊四氮（PTZ）（一种标准的致惊厥药物）诱导的 Wistar 大鼠肝和血清中的氧化损伤具有保护作用。动物（每组 16 只）通过灌胃给予生理盐水、有机葡萄汁或常规葡萄汁（10 μL/g 体重）17 天。随后，每组一半的大鼠接受 PTZ（60 mg/kg）。30 分钟后，动物被断头处死。分离肝脏和血液样本以评估氧化参数（脂质和蛋白质氧化、一氧化氮代谢物含量、抗氧化防御和蛋白质巯基含量）。这项研究的结果表明，尽管有机果汁的多酚含量高于常规果汁，但两种果汁都能防止脂质和蛋白质氧化损伤，并限制 PTZ 诱导的肝和血清中一氧化氮代谢物含量的增加。此外，两种果汁均抑制了 PTZ 诱导的肝和血清中酶抗氧化防御（超氧化物歧化酶和过氧化氢酶活性）和巯基蛋白含量的降低。综上所述，有机和常规葡萄汁均能减轻 Wistar 大鼠肝和血清中 PTZ 诱导的氧化损伤。
