高效液相色谱-柱后Ru(bipy)3(2+)-Ce(SO4)2化学发光检测法测定人血清和尿液样本中的水杨酸
Determination of salicylic acid in human serum and urine samples by high-performance liquid chromatography with post-column Ru(bipy)3(2+)-Ce(SO4)2 chemiluminescence detection.
作者信息
Long Xingyu, Chen Funan, Deng Mao
机构信息
School of Chemistry and Chemical Engineering of Southwest University, Chongqing, P. R. China.
出版信息
Anal Sci. 2013;29(2):227-31. doi: 10.2116/analsci.29.227.
A sensitive, novel and rapid chemiluminescence (CL) method combined with high-performance liquid chromatography (HPLC) separation for the determination of salicylic acid (SA) is described in this work. The method was based on the fact that SA could significantly enhance the CL of the reaction of cerium sulfate and the tris(2,2-bipyridyl) ruthenium(II) CL system in the presence of acid. Under the optimal conditions, the CL intensity was linear over concentrations of SA in the range of 0.02-10 × 10(-6) g/mL, with a detection limit of 8 × 10(-9) g/mL (S/N = 3). Also, the relative standard detection was 2.2% for 1.0 × 10(-7) g/mL (n = 11). The proposed method has been successfully applied to the analysis of SA in human serum samples and urine samples with satisfactory results.
本文描述了一种灵敏、新颖且快速的化学发光（CL）方法，该方法结合高效液相色谱（HPLC）分离用于测定水杨酸（SA）。该方法基于这样一个事实：在酸性条件下，SA能显著增强硫酸铈与三（2,2 - 联吡啶）钌（II）化学发光体系反应的化学发光。在最佳条件下，SA浓度在0.02 - 10×10⁻⁶ g/mL范围内时，化学发光强度呈线性关系，检测限为8×10⁻⁹ g/mL（S/N = 3）。此外，对于1.0×10⁻⁷ g/mL的SA，相对标准偏差为2.2%（n = 11）。所提出的方法已成功应用于人血清样品和尿液样品中SA的分析，结果令人满意。
Zotero 插件