利用 Ru(bipy) -Ce(SO ) 化学发光法测定牛奶和蛋清样品中的盐酸土霉素。

Determination of oxytetracycline hydrochloride in milk and egg white samples using Ru(bipy) -Ce(SO ) chemiluminescence.

机构信息

School of Chemistry and Materials Science, Guizhou Normal University, Guiyang, China.

Journal Editorial Department, Guizhou Normal University, Guiyang, China.

出版信息

Luminescence. 2019 May;34(3):316-323. doi: 10.1002/bio.3609. Epub 2019 Feb 5.

DOI:10.1002/bio.3609

PMID:30724008

Abstract

A novel, rapid and sensitive chemiluminescence (CL) method for the determination of oxytetracycline hydrochloride (OTCH) is described in this paper. The presented method was based on the fact that OTCH could immensely enhance the CL of the reaction of cerium sulfate and tris(2,2-bipyridyl) ruthenium (II) in acidic medium. Under optimal experimental conditions, CL intensity was favorably linear for OTCH in the range 5.0 × 10 to 5.0 × 10 g/ml, with a detection limit of 1.5 × 10 g/ml (S/N = 3). The relative standard detection was 4.76% for 5.0 × 10 g/ml (n = 11). This method was successfully applied to the analysis of OTCH in milk and egg white samples. According to the results of the kinetic curves for OTCH in the Ru(bipy) -Ce(SO ) CL system, together with CL and ultraviolet (UV)-visible spectra, the possible mechanism of the CL reaction is discussed briefly.

摘要

本文描述了一种测定盐酸土霉素（OTCH）的新型、快速、灵敏的化学发光（CL）方法。该方法基于 OTCH 能够极大增强硫酸铈和三（2,2-联吡啶）钌（II）在酸性介质中反应的 CL 这一事实。在最佳实验条件下，CL 强度与 OTCH 在 5.0×10 至 5.0×10 范围内呈良好线性关系，检测限为 1.5×10 （S/N=3）。对于 5.0×10 ，相对标准检测为 4.76%（n=11）。该方法成功应用于牛奶和蛋清样品中 OTCH 的分析。根据 OTCH 在 Ru（bipy）-Ce（SO ）CL 体系中的动力学曲线以及 CL 和紫外（UV）-可见光谱的结果，简要讨论了 CL 反应的可能机制。