Smits R P, Steinbusch H W, Mulder A H
Department of Pharmacology, Faculty of Medicine, Free University, Amsterdam, The Netherlands.
J Chem Neuroanat. 1990 Mar-Apr;3(2):101-23.
The distribution of dopamine (DA)-containing neurons in the guinea-pig brain was investigated immunohistochemically using antibodies raised against glutaraldehyde-conjugated DA. Light microscopical studies revealed the presence of nearly 50,000 DA-immunoreactive cells, localized throughout the hypothalamus and the midbrain. With a few exceptions the dopaminergic cell groups identified by different (immuno)histochemical techniques in the rat were also demonstrated in the guinea-pig brain. These include tyrosine hydroxylase-immunoreactive positive, dopamine-beta-hydroxylase-immunoreactive negative cells in the medial hypothalamus, the ventral tegmental area, the substantia nigra and the dorsal raphe nucleus. A group of dopaminergic cell bodies, not present in the rat, was found to extend from the nucleus retrochiasmaticus into the rostral part of the eminentia mediana. Also at variance with the rat, the guinea-pig lacked DA-immunoreactive cells in a number of homologous areas corresponding to the A15- and A8 cell groups and partly, the A14- and A10 cell groups. However, in general, the localization of dopaminergic cell bodies in the guinea-pig brain appeared similar to that in the rat brain.
利用针对戊二醛结合多巴胺产生的抗体，通过免疫组织化学方法研究了豚鼠脑中含多巴胺（DA）神经元的分布。光学显微镜研究显示，存在近50,000个DA免疫反应性细胞，分布于整个下丘脑和中脑。除少数例外，在大鼠中通过不同（免疫）组织化学技术鉴定的多巴胺能细胞群在豚鼠脑中也得到了证实。这些包括下丘脑内侧、腹侧被盖区、黑质和背侧中缝核中酪氨酸羟化酶免疫反应阳性、多巴胺-β-羟化酶免疫反应阴性的细胞。发现一组大鼠中不存在的多巴胺能细胞体，从视交叉后核延伸至中隆起的前部。与大鼠不同的是，豚鼠在一些与A15和A8细胞群相对应、部分与A14和A10细胞群相对应的同源区域缺乏DA免疫反应性细胞。然而，总体而言，豚鼠脑中多巴胺能细胞体的定位与大鼠脑中的相似。
