Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL.
J Biophotonics. 2014 Jan;7(1-2):96-102. doi: 10.1002/jbio.201200240. Epub 2013 Feb 8.
Bone-marrow (BM)-derived cells have been shown to be capable of aiding skin regeneration in vivo by differentiating into keratinocytes. However, the conditions under which this occurs are not fully understood. Characterizing innate mechanisms of skin regeneration by stem cells in vivo is important for the area of stem cell biology. In this study, we investigate the use of novel in vivo imaging technology for characterizing the contribution of BM-derived cells to regeneration of the epidermis in mouse skin in vivo. In vivo imaging provides the ability to non-invasively observe the spatial positions and morphology of the BM-derived cells. Using a GFP BM-transplanted mouse model and in vivo multimodal microscopy, BM-derived cells can be observed in the skin. Our in vivo imaging method was used to search for the presence and identify the 3D spatial distribution of BM-derived cells in the epidermis of the skin under normal conditions, following wound healing, and after syngeneic skin grafting. We did not observe any evidence of BM-derived keratinocytes under these conditions, but we did observe BM-derived dendritic cells in the skin grafts. In vivo multimodal imaging has great potential for characterizing the conditions under which BM-derived cells contribute to skin regeneration.
骨髓（BM）来源的细胞已被证明能够通过分化为角质形成细胞来帮助体内皮肤再生。然而，其发生的条件尚不完全清楚。体内干细胞对皮肤再生的固有机制进行特征描述对于干细胞生物学领域非常重要。在这项研究中，我们研究了使用新型体内成像技术来描述 BM 来源的细胞对体内小鼠皮肤表皮再生的贡献。体内成像提供了非侵入性地观察 BM 来源细胞的空间位置和形态的能力。使用 GFP BM 移植小鼠模型和体内多模态显微镜，可以观察到皮肤中的 BM 来源细胞。我们的体内成像方法用于在正常条件、伤口愈合后和同种异体皮肤移植后，搜索并识别表皮中 BM 来源细胞的存在和 3D 空间分布。在这些条件下，我们没有观察到任何 BM 来源的角质形成细胞的证据，但我们确实在皮肤移植物中观察到了 BM 来源的树突状细胞。体内多模态成像在描述 BM 来源的细胞对皮肤再生的贡献的条件方面具有巨大的潜力。
