Silveira-Lemos Denise, Fernandes Costa-Silva Matheus, Cardoso de Oliveira Silveira Amanda, Azevedo Batista Mauricio, Alves Oliveira-Fraga Lúcia, Soares Silveira Alda Maria, Barbosa Alvarez Maria Carolina, Martins-Filho Olindo Assis, Gazzinelli Giovanni, Corrêa-Oliveira Rodrigo, Teixeira-Carvalho Andréa
Laboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou, FIOCRUZ, Barro Preto, 30190-002 Belo Horizonte, MG, Brazil ; Laboratório de Imunologia Celular e Molecular, Centro de Pesquisas René Rachou, FIOCRUZ, 30190-002 Belo Horizonte, MG, Brazil ; Laboratório de Imunologia e Genômica de Parasitos, Departamento de Parasitologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil ; Laboratório de Imunoparasitologia, Departamento de Ciências Biológicas, NUPEB, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
J Parasitol Res. 2013;2013:909134. doi: 10.1155/2013/909134. Epub 2013 Jan 20.
Acute schistosomiasis is associated with a primary exposure and is more commonly seen in nonimmune individuals traveling through endemic regions. In this study, we have focused on the cytokine profile of T lymphocytes evaluated in circulating leukocytes of acute Schistosomiasis mansoni-infected patients (ACT group) before and after praziquantel treatment (ACT-TR group). Our data demonstrated increased values of total leukocytes, eosinophils, and monocytes in both groups. Interestingly, we have observed that patients treated with praziquantel showed increased values of lymphocytes as compared with noninfected group (NI) or ACT groups. Furthermore, a decrease of neutrophils in ACT-TR was observed when compared to ACT group. Analyses of short-term in vitro whole blood stimulation demonstrated that, regardless of the presence of soluble Schistosoma mansoni eggs antigen (SEA), increased synthesis of IFN-γ and IL-4 by T-cells was observed in the ACT group. Analyses of cytokine profile in CD8 T cells demonstrated higher percentage of IFN-γ and IL-4 cells in both ACT and ACT-TR groups apart from increased percentage of IL-10 cells only in the ACT group. This study is the first one to point out the relevance of CD8 T lymphocytes in the immune response induced during the acute phase of schistosomiasis.
急性血吸虫病与初次接触有关，在前往流行地区的非免疫个体中更为常见。在本研究中，我们重点关注了在曼氏血吸虫急性感染患者（ACT组）吡喹酮治疗前后（ACT-TR组）循环白细胞中评估的T淋巴细胞细胞因子谱。我们的数据显示两组的总白细胞、嗜酸性粒细胞和单核细胞值均升高。有趣的是，我们观察到与未感染组（NI）或ACT组相比，接受吡喹酮治疗的患者淋巴细胞值升高。此外，与ACT组相比，ACT-TR组中性粒细胞减少。短期体外全血刺激分析表明，无论是否存在可溶性曼氏血吸虫卵抗原（SEA），ACT组T细胞中IFN-γ和IL-4的合成均增加。CD8 T细胞细胞因子谱分析表明，ACT组和ACT-TR组中IFN-γ和IL-4细胞的百分比均较高，而仅ACT组中IL-10细胞的百分比增加。本研究首次指出CD8 T淋巴细胞在血吸虫病急性期诱导的免疫反应中的相关性。
