Mduluza Takafira, Mutapi Francisca, Ruwona Tinashe, Kaluka Daniel, Midzi Nicholas, Ndhlovu Patricia D
Department of Biochemistry, University of Zimbabwe.
Malawi Med J. 2009 Dec;21(4):176-82. doi: 10.4314/mmj.v21i4.49642.
The effect of treatment with either oxamniquine or praziquantel on S.mansoni specific IFN-gamma, IL-4, IL-5 and IL-10 was compared on PBMC which were collected pretreatment, 6 and 18 weeks post treatment. Using sandwich ELISA on the supernatants harvested from the PBMC stimulation by crude S. mansoni SEA and SWAP antigens after 5 days the levels of PBMC proliferation and cytokine production were similar according to treatment with either praziquantel or oxamniquine. Before treatment, infected groups showed low ratios, of IL-4:IFN-gamma, IL-5:IFNgamma and IL-10:IFN-gamma, indicating that IFN-gamma was high in the infected individuals. The general increase in immuno-modulation was observed post-treatment with elevated immune reactivity and cytokine production in both treatment groups. Treatment induced significant increases in levels of IL-4 (p < 0.05), IL-5 (p < 0.0001) and IL-10 (p < 0.05) cytokines 6 and 18 weeks after treatment. There were no significant differences in the increase in IL-4, IL-5 and IL-10 between children treated with praziquantel or oxamniquine. Pre-treatment IFN-gamma and IL-5 levels were positively correlated with infection (p < 0.001), while post treatment IL-4 cytokine levels were negatively correlated with baseline infection status (p < 0.001). The results suggest that treatment-induced immune responses are similar for both common anti-schistosome drugs praziquantel or oxamniquine having similar and immunizing effect.
比较了用奥沙尼喹或吡喹酮治疗对曼氏血吸虫特异性干扰素-γ、白细胞介素-4、白细胞介素-5和白细胞介素-10的影响，这些指标是在治疗前、治疗后6周和18周采集的外周血单个核细胞（PBMC）上进行检测的。通过夹心酶联免疫吸附测定法（ELISA）检测经曼氏血吸虫粗制可溶性虫卵抗原（SEA）和可溶性成虫抗原（SWAP）刺激5天后PBMC上清液中的细胞因子水平，结果显示，吡喹酮或奥沙尼喹治疗后PBMC增殖和细胞因子产生水平相似。治疗前，感染组的白细胞介素-4:干扰素-γ、白细胞介素-5:干扰素-γ和白细胞介素-10:干扰素-γ比值较低，表明感染个体中干扰素-γ水平较高。治疗后观察到免疫调节普遍增强，两个治疗组的免疫反应性和细胞因子产生均升高。治疗后6周和18周，治疗诱导白细胞介素-4（p < 0.05）、白细胞介素-5（p < 0.0001）和白细胞介素-10（p < 0.05）细胞因子水平显著升高。吡喹酮或奥沙尼喹治疗的儿童在白细胞介素-4、白细胞介素-5和白细胞介素-10升高方面无显著差异。治疗前干扰素-γ和白细胞介素-5水平与感染呈正相关（p < 0.001），而治疗后白细胞介素-4细胞因子水平与基线感染状态呈负相关（p < 0.001）。结果表明，两种常用抗血吸虫药物吡喹酮或奥沙尼喹诱导的免疫反应相似，具有相似的免疫效果。
