Tret'iakova I N, Barsukova A V
Ontogenez. 2012 Nov-Dec;43(6):425-35.
Embryogenic callus formation in different larch species from Siberia (Larix sibirica, L. gmelinii, and L. sukaczewii) was carried out on MSGm medium supplemented with growth regulators (2.4-D and BAP) and followed one and the same scheme: elongation of somatic cells and their asymmetric division with formation of initial and tube cells. The cells of embryo initial underwent sequential divisions and formed embryonic globules which caused the formation of somatic embryos. Somatic embryos became mature and germinated by addition of ABA and PEG into the medium. Long-term proliferating cell lines and regenerant plants were obtained in Sukachev larch and its hybrid with Siberian larch. The success of somatic embryogenesis depended on the genotype of the donor tree.
对来自西伯利亚的不同落叶松物种（西伯利亚落叶松、兴安落叶松和苏氏落叶松）进行胚性愈伤组织诱导，在添加了生长调节剂（2,4-二氯苯氧乙酸和苄氨基嘌呤）的MSGm培养基上进行，遵循相同的方案：体细胞伸长并进行不对称分裂，形成原始细胞和管细胞。胚原始细胞进行连续分裂，形成胚性球状体，进而导致体细胞胚的形成。通过向培养基中添加脱落酸和聚乙二醇，体细胞胚成熟并萌发。在苏氏落叶松及其与西伯利亚落叶松的杂交种中获得了长期增殖的细胞系和再生植株。体细胞胚胎发生的成功取决于供体树的基因型。
