Verma Manju, Bansal Y K
Plant Tissue Culture Laboratory, Department of P.G. Studies and Research in Biological Science, R. D University, Jabalpur 482 001, India.
Indian J Exp Biol. 2012 Dec;50(12):904-9.
An efficient protocol has been developed for regeneration of complete plants through somatic embryogenesis in H. coronarium. Creamish white, pale yellow and brown calli were obtained on MS medium supplemented with different concentrations of auxins [2, 4-Dichlorophenoxy acetic acid (2, 4-D), Indole-3 acetic acid (IAA) and 1-Naphthylacetic acid (NAA)] after 4 weeks. Creamy white calli developed on 0.5 mg L(-1) 2, 4-D turned embryogenic when subcultured on basal medium and produced small globular somatic embryos after 6 weeks. Further growth of somatic embryos required their transfer to medium containing 6-benzylaminopurine (BAP) or kinetin (KN). BAP was more effective than KN in promoting shoot proliferation. Maximum shoot length was obtained with 0.5 mg L(-1) BAP whereas maximum shoot number was obtained with 1.0 mg L(-1) BAP. The plantlets thus formed were successfully hardened, and transferred to sand-soil and farm yard manure (1:1:1) with 95% survival.
已开发出一种高效方案，用于通过冠状银合欢的体细胞胚胎发生再生完整植株。4周后，在添加不同浓度生长素[2,4-二氯苯氧乙酸（2,4-D）、吲哚-3-乙酸（IAA）和1-萘乙酸（NAA）]的MS培养基上获得了乳白、淡黄和褐色愈伤组织。在0.5 mg L(-1) 2,4-D上形成的乳白愈伤组织在基础培养基上继代培养时变为胚性，6周后产生小的球形体细胞胚。体细胞胚的进一步生长需要将其转移到含有6-苄基腺嘌呤（BAP）或激动素（KN）的培养基上。BAP在促进芽增殖方面比KN更有效。0.5 mg L(-1) BAP时获得最大芽长，而1.0 mg L(-1) BAP时获得最大芽数。如此形成的小植株成功炼苗，并转移到沙-土和农家肥（1:1:1）中，成活率达95%。
