Key Laboratory of Industrial Ecology and Environmental Engineering (China Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, China.
Bioresour Technol. 2013 Mar;131:527-30. doi: 10.1016/j.biortech.2013.01.099. Epub 2013 Jan 29.
Graphene oxide (GO) was applied in this study to enhance the activity of anaerobic ammonium oxidation (anammox) bacteria for nitrogen removal. A GO dose-dependent effect on anammox bacteria was observed through batch tests. The results showed that the activity increased as the GO dose was varied within 0.05-0.1gL(-1). A maximum 10.26% increase of anaerobic ammonium oxidizing activity was achieved at 0.1gL(-1) GO. Analysis of extracellular polymeric substances (EPS) indicated that the highest carbohydrate, protein, and total EPS contents (42.5, 125.7, and 168.2mg (g volatile suspended solids)(-1), respectively) were obtained with 0.1gL(-1) GO. Appropriate GO dose stimulated EPS production to promote the activity of anammox bacteria. Transmission electron microscopy showed the large surface area of GO benefited cell attachment. These findings proved that the application of GO was an effective approach to enhancing the activity of anammox bacteria.
本研究采用氧化石墨烯（GO）来提高厌氧氨氧化菌的活性，以实现氮的去除。通过批量试验观察到 GO 对厌氧氨氧化菌具有剂量依赖性影响。结果表明，在 0.05-0.1gL(-1) 的范围内，随着 GO 剂量的变化，活性增加。在 0.1gL(-1)GO 时，厌氧氨氧化活性最大提高了 10.26%。对胞外聚合物（EPS）的分析表明，在 0.1gL(-1)GO 时获得了最高的碳水化合物、蛋白质和总 EPS 含量（分别为 42.5、125.7 和 168.2mg(g 挥发性悬浮固体)(-1)）。适当的 GO 剂量刺激 EPS 的产生，从而促进厌氧氨氧化菌的活性。透射电子显微镜显示，GO 的大表面积有利于细胞附着。这些发现证明了 GO 的应用是增强厌氧氨氧化菌活性的有效方法。
