Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, PR China.
Bioresour Technol. 2011 Mar;102(5):4290-3. doi: 10.1016/j.biortech.2010.12.050. Epub 2010 Dec 22.
In this study, effect of low intensity ultrasound on the activity of anammox microbial consortium for nitrogen removal was investigated through batch experiments at the same irradiation frequency of 25 kHz. Total nitrogen removal rate increased by about 25.5% when ultrasound intensity of 0.3 w cm(-2) was applied at an optimal irradiation time of 4 min, and further experiments demonstrated that this effect could last for about 6 days. Analysis of extracellular polymeric substances indicated that the maximum increase of carbohydrate, protein and total extracellular substances was obtained on the first day after ultrasound, which was 28.8%, 30.5% and 29.7%, respectively. As the time prolonged, the production rate of extracellular carbohydrate, protein decreased gradually. Transmission electron microscopy observation demonstrated that ultrasounded cell wall of anammox microbial consortium became thinner resulting in increased release of extracellular substances. The results suggested that application of low intensity ultrasound may enhance the activity of anammox microbial consortium and ultimately the potential for nitrogen removal.
在这项研究中，通过在相同的 25 kHz 辐照频率下进行批处理实验，研究了低强度超声对厌氧氨氧化微生物共混物脱氮活性的影响。当超声强度为 0.3 w cm(-2)，辐照时间为 4 分钟时，总氮去除率提高了约 25.5%，进一步的实验表明，这种效果可以持续约 6 天。对胞外聚合物的分析表明，在超声后的第一天，碳水化合物、蛋白质和总胞外物质的最大增加量分别为 28.8%、30.5%和 29.7%。随着时间的延长，胞外碳水化合物、蛋白质的产生率逐渐下降。透射电子显微镜观察表明，超声处理后的厌氧氨氧化微生物共混物细胞壁变薄，导致胞外物质的释放增加。结果表明，低强度超声的应用可以增强厌氧氨氧化微生物共混物的活性，从而提高脱氮的潜力。
