FASN 的遗传变异与韩牛脂肪酸组成有关。

Genetic variation of FASN is associated with fatty acid composition of Hanwoo.

机构信息

National Institute of Animal Science, Pyeongchang, Republic of Korea.

出版信息

Meat Sci. 2013 May;94(1):133-8. doi: 10.1016/j.meatsci.2013.01.002. Epub 2013 Jan 17.

DOI:10.1016/j.meatsci.2013.01.002

Abstract

The objective of this study was to identify genetic variants in the bovine fatty acid synthase (FASN) gene and to evaluate associations with fatty acid composition from longissimus lumborum muscle using 90 purebred Hanwoo steers. Sequence alignments observed 6 genetic variants located in exons 20, 24, 32, 34, and 39, and PCR-RFLP analysis confirmed these variations. Genotypes of the g.15532A>C locus were significantly associated with Linolenic acid (C18:3), and genotypes of the g.17924G>A locus were significantly associated with Palmitic (C16:0), Palmitoleic (C16:1), Oleic (C18:1), saturated fatty acids, and unsaturated fatty acids. The analysis revealed that SFA and UFA showed significant correlations with fatty acid composition (Myristic (C14:0), Palmitic (C16:0), Stearic (C18:0), Oleic (C18:1), and Eicosenoic (C20:1) acids). Oleic acid (C18:1) was negatively correlated with Myristic (C14:0), Palmitic (C16:0), and Palmitoleic (C16:1) acids (P<0.001).

摘要

本研究旨在鉴定牛脂肪酸合酶（FASN）基因中的遗传变异，并使用 90 头纯种韩牛公牛评估其与背最长肌脂肪酸组成的关联。序列比对观察到 6 个位于外显子 20、24、32、34 和 39 的遗传变异，PCR-RFLP 分析证实了这些变异。g.15532A>C 位点的基因型与亚麻酸（C18:3）显著相关，g.17924G>A 位点的基因型与棕榈酸（C16:0）、棕榈油酸（C16:1）、油酸（C18:1）、饱和脂肪酸和不饱和脂肪酸显著相关。分析表明 SFA 和 UFA 与脂肪酸组成呈显著相关（豆蔻酸（C14:0）、棕榈酸（C16:0）、硬脂酸（C18:0）、油酸（C18:1）和二十碳烯酸（C20:1））。油酸（C18:1）与豆蔻酸（C14:0）、棕榈酸（C16:0）和棕榈油酸（C16:1）呈负相关（P<0.001）。