Przybylska Paulina, Kuczaj Marian
Institute of Animal Breeding, Wroclaw University of Environmental and Life Sciences, ul. Chelmonskiego 38C, 50-375 Wroclaw, Poland.
Animals (Basel). 2024 Aug 4;14(15):2268. doi: 10.3390/ani14152268.
Fatty acid synthase (FASN) is a metabolic enzyme responsible for the synthesis of fatty acids in milk and meat. The SNPs g.841G/C and g.17924A/G of the FASN gene significantly influence the fat and fatty acid content of milk from cows of various breeds. Therefore, these SNPs were selected for this study. This study aimed to analyze the relationship of SNPs and their genotypes with the fat content and fatty acid profile of milk from Polish Red-and-White (ZR), Polish Red (RP), and Polish Holstein-Friesian Red-and-White (RW) cows. Milk samples were obtained during a milking trial. SNP genotyping was performed using the real-time PCR (HRM) method. It was shown that SNPs (with specific genotypes) were significantly associated with the presence of fatty acids such as C18:1n9t and C18:2n6c in milk. In addition, it was found that the milk fat from the ZR (genotypic variant A/G, AA) and RP (genotypic variant GG, A/G) breeds often exhibited a more attractive fatty acids profile than the milk fat from RW cows. This information can be used by both cattle breeders and people interested in consuming functional foods.
脂肪酸合酶（FASN）是一种代谢酶，负责牛奶和肉类中脂肪酸的合成。FASN基因的单核苷酸多态性（SNPs）g.841G/C和g.17924A/G显著影响不同品种奶牛所产牛奶的脂肪和脂肪酸含量。因此，本研究选择了这些SNPs。本研究旨在分析波兰红白花牛（ZR）、波兰红牛（RP）和波兰荷斯坦-弗里生红白花牛（RW）所产牛奶中SNPs及其基因型与脂肪含量和脂肪酸谱之间的关系。在一次挤奶试验中采集了牛奶样本。使用实时聚合酶链反应（HRM）方法进行SNP基因分型。结果表明，SNPs（具有特定基因型）与牛奶中C18:1n9t和C18:2n6c等脂肪酸的存在显著相关。此外，还发现ZR（基因型变体A/G、AA）和RP（基因型变体GG、A/G）品种的牛奶脂肪通常比RW奶牛的牛奶脂肪呈现出更具吸引力的脂肪酸谱。养牛者和对食用功能性食品感兴趣的人都可以利用这些信息。
