胰蛋白酶大豆培养基适用于原位制备含有少量特定数量微生物细胞的标准品。
Tryptic soy medium is feasible for the in situ preparation of standards containing small defined numbers of microbial cells.
机构信息
Tokyo University of Agriculture and Technology, Department of Biotechnology and Life Science, 2-24-16 Naka-cho, Koganei, Tokyo, Japan.
出版信息
J Microbiol Methods. 2013 Apr;93(1):49-51. doi: 10.1016/j.mimet.2013.01.021. Epub 2013 Feb 10.
PMID:23403310
Abstract
A standard material comprising a few viable cells of a microorganism is essential for rational validation of microbiological methods. We propose a method of a flow cytometric sorting of cells stained with 6-carboxyfluorescein diacetate. The feasibility of tryptic soy medium in this method is demonstrated with 5 strains.
摘要
标准物质应由少数活的微生物细胞组成，这对于微生物方法的合理验证至关重要。我们提出了一种用 6-羧基荧光素二乙酸酯染色的细胞进行流式细胞术分选的方法。该方法采用 5 株菌验证了胰蛋白胨大豆培养基的可行性。
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