Tokyo University of Agriculture and Technology, Department of Biotechnology and Life Science, 2-24-16 Naka-cho, Koganei, Tokyo, Japan.
J Microbiol Methods. 2013 Apr;93(1):49-51. doi: 10.1016/j.mimet.2013.01.021. Epub 2013 Feb 10.
A standard material comprising a few viable cells of a microorganism is essential for rational validation of microbiological methods. We propose a method of a flow cytometric sorting of cells stained with 6-carboxyfluorescein diacetate. The feasibility of tryptic soy medium in this method is demonstrated with 5 strains.
标准物质应由少数活的微生物细胞组成，这对于微生物方法的合理验证至关重要。我们提出了一种用 6-羧基荧光素二乙酸酯染色的细胞进行流式细胞术分选的方法。该方法采用 5 株菌验证了胰蛋白胨大豆培养基的可行性。
