Institute of Biological Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, United Kingdom.
Université Bourgogne Franche-Comté, AgroSup Dijon, Dijon, France.
Curr Protoc Cytom. 2020 Jun;93(1):e72. doi: 10.1002/cpcy.72.
For microorganisms in particular, viability is a term that is difficult to define and a state consequently difficult to measure. The traditional (and gold standard) usage equates viability and culturability (i.e., the ability to multiply) but the process of determining culturability is often too slow. Flow cytometry provides the opportunity to make rapid and quantitative measurements of dye uptake in large numbers of cells and we can therefore exploit the flow cytometric approach to evaluate so-called viability stains and to develop protocols for more routine assessments of microbial viability. This article provides a commentary and several protocols have been included to ensure that users have a firm basis for attempting these reasonably difficult assays on traditional flow cytometer instruments. What is clear is that each assay must be carefully validated with the particular microorganism of interest before being applied in any research, clinical, or service form. © 2020 The Authors.
对于微生物来说，生存能力是一个难以定义的术语，因此也是一个难以衡量的状态。传统的（也是黄金标准的）用法将生存能力和可培养性（即繁殖能力）等同起来，但可培养性的确定过程往往太慢。流式细胞术提供了快速定量测量大量细胞染料摄取的机会，因此我们可以利用流式细胞术方法来评估所谓的生存力染色，并开发更常规的微生物生存力评估方案。本文提供了一篇评论，并包含了几个方案，以确保用户在尝试使用传统流式细胞仪仪器进行这些相当困难的测定时有一个坚实的基础。很明显，在任何研究、临床或服务形式中应用之前，每个测定都必须与特定的感兴趣的微生物仔细验证。
