Leclerc P, Sirard M A, Chafouleas J G, Lambert R D
Ontogénie et Reproduction, Centre de Recherches du CHUL, Ste-Foy, Québec, Canada.
Biol Reprod. 1990 Mar;42(3):483-9. doi: 10.1095/biolreprod42.3.483.
The 125I-calmodulin gel overlay procedure was used to evaluate the effect of a heparin treatment on the calmodulin-binding proteins of bull spermatozoa. At concentrations that increase the in vitro fertilization rate of in vitro-matured oocytes, heparin induced a decrease in the binding to calmodulin (CaM) in 3 sperm proteins of 28, 30, and 49 kDa. The binding of these proteins to CaM was higher when Ca2+ was absent from the overlay procedure, and this binding was negatively correlated to the fertilization rate. These results suggest that sperm capacitation is associated with a decrease in the binding of CaM to the 28, 30, and 49 kDa sperm CaM-binding proteins. Implications of such a decrease are discussed.
采用¹²⁵I-钙调蛋白凝胶覆盖法评估肝素处理对公牛精子钙调蛋白结合蛋白的影响。在能提高体外成熟卵母细胞体外受精率的浓度下，肝素导致28、30和49 kDa的3种精子蛋白与钙调蛋白（CaM）的结合减少。当覆盖过程中不存在Ca²⁺时，这些蛋白与CaM的结合更高，且这种结合与受精率呈负相关。这些结果表明，精子获能与CaM与28、30和49 kDa精子CaM结合蛋白的结合减少有关。讨论了这种减少的影响。
