Departamento de Microbiologia, Instituto de Ciências Biomédicas II, Universidade de São Paulo (USP), São Paulo, SP, Brazil.
Mycopathologia. 2013 Apr;175(3-4):231-9. doi: 10.1007/s11046-013-9616-5. Epub 2013 Feb 13.
Opportunistic infections are an increasingly common problem in hospitals, and the yeast Candida parapsilosis has emerged as an important nosocomial pathogen. The aims of this study were to determine and compare (i) the prevalence rate among C. parapsilosis complex organisms isolated from blood in a public children's hospital in São Paulo state, (ii) the ability of the complex C. parapsilosis species identified to produce biofilm and (iii) the antifungal susceptibility profiles. Forty-nine (49) specimens of isolated blood yeast were analyzed, previously identified as C. parapsilosis by conventional methods. After the molecular analysis, the isolates were characterized as C. parapsilosis sensu stricto (83.7 %), C. orthopsilosis (10.2 %) and C. metapsilosis (6.1 %). All species were able to form biofilm. The species with the highest biofilm production was C. parapsilosis sensu stricto, followed by C. orthopsilosis and further by C. metapsilosis. All of the strains have demonstrated similar susceptibility to fluconazole, caspofungin, voriconazole, cetoconazole and 5-flucytosine. Only one strain of C. parapsilosis was resistant to amphotericin B. Regarding itraconazole, 66.6 and 43.9 % isolates of C. metapsilosis and C. parapsilosis, respectively, have demonstrated to be susceptible dose-dependent, with one isolate of the latter species resistant to the drug. Candida parapsilosis sensu stricto has demonstrated to be the less susceptible, mainly to amphotericin B, caspofungin and "azoles" such as fluconazole. Therefore, C. metapsilosis and C. orthopsilosis are still involved in a restricted number of infections, but these data have become essential for there are very few studies of these species in Latin America.
机会性感染是医院中越来越常见的问题，而假丝酵母近平滑念珠菌已成为一种重要的医院获得性病原体。本研究的目的是确定和比较（i）在圣保罗州一家公立儿童医院分离的近平滑念珠菌复合体血液分离株的流行率，（ii）鉴定出的近平滑念珠菌复合体种产生生物膜的能力，以及（iii）抗真菌药敏谱。分析了 49 份分离的血液酵母标本，这些标本先前通过常规方法鉴定为假丝酵母近平滑念珠菌。在进行分子分析后，将分离株鉴定为近平滑念珠菌（83.7%）、近平滑念珠菌（10.2%）和中间型念珠菌（6.1%）。所有种都能够形成生物膜。产膜能力最强的种是近平滑念珠菌，其次是近平滑念珠菌和中间型念珠菌。所有菌株对氟康唑、卡泊芬净、伏立康唑、酮康唑和 5-氟胞嘧啶均具有相似的敏感性。只有一株假丝酵母近平滑念珠菌对两性霉素 B 耐药。关于伊曲康唑，66.6%和 43.9%的中间型念珠菌和假丝酵母近平滑念珠菌的分离株对其表现为敏感剂量依赖性，其中一株假丝酵母近平滑念珠菌对该药物耐药。近平滑念珠菌的敏感性最低，主要对两性霉素 B、卡泊芬净和氟康唑等“唑类”药物耐药。因此，中间型念珠菌和近平滑念珠菌仍然涉及到少数感染，但这些数据对于拉丁美洲这些种的研究非常少来说是必不可少的。
