中国仓鼠细胞系中的叶酸敏感脆性位点。

Folate-sensitive fragile sites in Chinese hamster cell lines.

作者信息

Simi S, Vatteroni L, Piras A, Mariani T, Rainaldi G

机构信息

Genetica e Biochimica delle Cellule Somatiche, Istituto di Mutagenesi e Differenziamento CNR, Pisa, Italy.

出版信息

Cancer Genet Cytogenet. 1990 Jun;46(2):209-16. doi: 10.1016/0165-4608(90)90106-k.

DOI:10.1016/0165-4608(90)90106-k

PMID:2340492

Abstract

The expression of fragile sites in three different Chinese hamster cell lines was studied. Results showed that folate-sensitive fragile sites were expressed in the pericentromeric regions of chromosomes 1, 3, 4, 6, and 7 and in band 1q22. A comparison of the breakpoints involved in formation of chromosome rearrangements in some established Chinese hamster cell lines was also made. Results showed that while the specific type of rearrangement was random, the breakpoints were not. Three of the chromosomal sites most frequently involved in breaks were regions in which fragile sites were expressed.

摘要

对三种不同的中国仓鼠细胞系中脆性位点的表达进行了研究。结果表明，叶酸敏感脆性位点在1、3、4、6和7号染色体的着丝粒周围区域以及1q22带中表达。还对一些已建立的中国仓鼠细胞系中染色体重排形成所涉及的断点进行了比较。结果表明，虽然重排的具体类型是随机的，但断点并非如此。最常发生断裂的三个染色体位点是脆性位点表达的区域。