姐妹染色单体交叉能否被视为前期损伤？

Can sister chromatid intercrossings be considered as prelesions?

作者信息

Fuster C, Mirò R, Templado C, Barrios L, Egozcue J

机构信息

Departament de Biologia Cel.lular i Fisiologia, Facultat de Medicina, Autònoma de Barcelona, Spain.

出版信息

Hum Genet. 1988 Jun;79(2):179-80. doi: 10.1007/BF00280561.

DOI:10.1007/BF00280561

PMID:3391616

Abstract

In a study of the expression of folate-sensitive fragile sites in five normal individuals using RPMI medium containing methotrexate (MTX), we observed a high frequency of "sister chromatid intercrossings" (SCI) that is, the intersection of sister chromatids. The location of SCIs corresponded to fragile sites in 54.2% of the cases. Of the SCIs observed in each individual, 43%-53% were located at the same bands as their expressed fragile sites. Furthermore when RPMI + MTX medium was used instead of F-10 medium, the incidence of SCIs increased tenfold. We suggest that SCIs could indicate the existence of a pre-lesion.

摘要

在一项使用含甲氨蝶呤（MTX）的RPMI培养基对五名正常个体叶酸敏感性脆性位点表达的研究中，我们观察到了高频率的“姐妹染色单体交叉”（SCI），即姐妹染色单体的交叉。SCI的位置在54.2%的病例中与脆性位点相对应。在每个个体中观察到的SCI中，43%-53%位于与其表达的脆性位点相同的条带处。此外，当使用RPMI+MTX培养基而非F-10培养基时，SCI的发生率增加了十倍。我们认为SCI可能表明存在一种前期病变。