家猪（野猪）染色体中的叶酸敏感常见脆性位点。

Folate sensitive common fragile sites in chromosomes of the domestic pig (Sus scrofa).

作者信息

Yang M Y, Long S E

机构信息

Department of Animal Husbandry, University of Bristol.

出版信息

Res Vet Sci. 1993 Sep;55(2):231-5. doi: 10.1016/0034-5288(93)90086-u.

DOI:10.1016/0034-5288(93)90086-u

PMID:8235092

Abstract

Chromosomal fragile sites were studied in blood lymphocytes of the pig after induction with 0, 5, 10 and 20 micrograms of fluorodeoxyuridine (FudR) ml-1 of culture. The percentage of cells showing fragile sites and total breaks and gaps in 100 cells was significantly higher in all treated cultures than the control (P < 0.001). A concentration of 5 to 10 micrograms of FudR ml-1 of medium is recommended as the optimum for mitotic index and fragile site expression in the pig. Localisation of the fragile sites was by GTG banding after destaining of the conventionally stained Giemsa preparations. Common fragile sites in the pig were identified in 24 sites on 10 chromosomes. Some fragile sites corresponded to the breakpoints in known reciprocal translocations.

摘要

在用每毫升培养基中含0、5、10和20微克氟脱氧尿苷（FudR）诱导后，对猪的血液淋巴细胞中的染色体脆性位点进行了研究。在所有处理过的培养物中，显示脆性位点以及100个细胞中的总断裂和间隙的细胞百分比均显著高于对照组（P < 0.001）。推荐每毫升培养基中含5至10微克FudR的浓度作为猪有丝分裂指数和脆性位点表达的最佳浓度。在对常规染色的吉姆萨制剂进行脱色后，通过GTG显带对脆性位点进行定位。在猪的10条染色体上的24个位点鉴定出了常见的脆性位点。一些脆性位点与已知相互易位中的断点相对应。