Vaillant C, Horadagoda N U, Batt R M
Department of Veterinary Preclinical Sciences, University of Liverpool, United Kingdom.
Cell Tissue Res. 1990 Apr;260(1):117-22. doi: 10.1007/BF00297496.
A cobalamin (vitamin B12)-binding protein has recently been identified in canine pancreatic juice which is biochemically, immunochemically and functionally similar to canine gastric intrinsic factor. However, the cellular sources of both this pancreatic intrinsic factor and gastric intrinsic factor in the dog are not known. Antisera raised against canine gastric intrinsic factor have been used to examine the distribution of intrinsic factors in the canine pancreas and stomach. Immunoreactivity was demonstrated in duct cells but not acinar or endocrine cells in the pancreas, and in fundic peptic and pyloric gastric pit cells in stomach. All immunostaining was abolished by preabsorption of the antisera with purified canine gastric and pancreatic intrinsic factors. A cellular source of pancreatic intrinsic factor has not been previously described, and the demonstration of intrinsic factor-like immunoreactivity in two cell types in the canine stomach contrasts with its localization in a single cell type in the gastric mucosa of other mammalian species. Furthermore, immunoreactivity in pancreatic duct cells was detected at much higher dilutions of antisera than those required for staining of peptic and gastric pit cells. This suggests a higher concentration of antigen, and supports previous evidence that the pancrease is a major source of intrinsic factor in the dog.
最近在犬胰液中发现了一种钴胺素（维生素B12）结合蛋白，它在生化、免疫化学和功能上与犬胃内因子相似。然而，犬体内这种胰腺内因子和胃内因子的细胞来源尚不清楚。用针对犬胃内因子产生的抗血清来检测内因子在犬胰腺和胃中的分布。在胰腺的导管细胞中显示有免疫反应性，而腺泡细胞或内分泌细胞中没有，在胃的胃底主细胞和幽门胃小凹细胞中也有免疫反应性。用纯化的犬胃和胰腺内因子预先吸收抗血清后，所有免疫染色均消失。胰腺内因子的细胞来源此前尚未见报道，犬胃中两种细胞类型出现内因子样免疫反应性，这与其他哺乳动物胃黏膜中内因子定位于单一细胞类型形成对比。此外，检测到胰腺导管细胞中的免疫反应性所需的抗血清稀释度比胃主细胞和胃小凹细胞染色所需的稀释度高得多。这表明抗原浓度更高，并支持了之前的证据，即胰腺是犬体内内因子的主要来源。
