Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.
Int J Biol Macromol. 2013 Oct;61:36-42. doi: 10.1016/j.ijbiomac.2013.06.035. Epub 2013 Jun 29.
This study was undertaken to develop an optimum composition model for the microencapsulation of a newly probiotic on sodium alginate using response surface methodology. The individual and interactive effects of three independent variables, namely sodium alginate concentration, biomass concentration, and hardening time, were investigated using Box-Behnken design experiments. A second ordered polynomial model was fitted and optimum conditions were estimated. The optimal conditions identified were 2% for sodium alginate, 10(10)UFC/ml for biomass, and 30 min for hardening time. The experimental value obtained for immobilized cells under these conditions was about 80.98%, which was in close agreement with the predicted value of 82.6%. Viability of microspheres (96%) was enhanced with chitosan as coating materials. The survival rates of free and microencapsulated Lactobacillus plantarum TN8 during exposure to artificial gastrointestinal conditions were compared. The results revealed that the encapsulated cells exhibited significantly higher resistances to artificial intestinal juice (AIJ) and artificial gastric juice (AGJ). Microencapsulation was also noted to effectively protect the strain from heating at 65 °C and refrigerating at 4 °C. Taken together, the findings indicated that microencapsulation conferred important protective effects to L. plantarum against the gastrointestinal conditions encountered during the transit of food.
本研究采用响应面法，对新益生菌的海藻酸钠微胶囊化进行了最佳组成模型的开发。使用 Box-Behnken 设计实验，研究了三个独立变量（海藻酸钠浓度、生物量浓度和硬化时间）的个体和交互作用。拟合了二次多项式模型，并估算了最佳条件。确定的最佳条件为海藻酸钠 2%、生物质 10(10)UFC/ml 和硬化时间 30 分钟。在这些条件下，固定化细胞的实验值约为 80.98%，与预测值 82.6%非常接近。使用壳聚糖作为包衣材料可以提高微球的存活率（96%）。比较了游离和微囊化植物乳杆菌 TN8 在暴露于人工胃肠条件下的存活率。结果表明，包封细胞对人工肠液（AIJ）和人工胃液（AGJ）表现出明显更高的抵抗力。微囊化还可以有效保护菌株免受 65°C 加热和 4°C 冷藏的影响。总之，这些发现表明，微囊化对植物乳杆菌在食物转运过程中遇到的胃肠条件具有重要的保护作用。
