LEITAT Technological Center , C/de la Innovació 2. 08225 Terrassa (Barcelona) , Spain.
Nanotoxicology. 2014 May;8(3):279-94. doi: 10.3109/17435390.2013.776123. Epub 2013 Mar 11.
Little information exists on the toxicological hazards associated to organo-modified clays. We evaluated the cytotoxicity of a series of pristine and organo-modified nanoclays in different cell lines. The calculated IC50 values for cell viability ranged from 1.4 to 47 µg/mL for the six organoclays used and were above 100 µg/mL for the pristine nanoclays. The IC50 values of the organoclays were driven by the proportion and structure of the quaternary ammonium compound used as surface organic modifier. No differences in cell toxicity were observed between the large and small-sized (additional milling step) nanoclay batches, although their size differences related mostly to upper range of the size distribution. Despite their lower toxicity, pristine nanoclays induced apoptosis and were found in cytoplasmic vesicles of exposed cells. Organoclays were also found in cytoplasmic vesicles, although the size of the agglomerates was larger and the efficiency of uptake was considerably lower.
关于有机改性粘土相关的毒理学危害的信息有限。我们评估了一系列原始和有机改性纳米粘土在不同细胞系中的细胞毒性。对于所使用的六种有机粘土，细胞活力的计算 IC50 值范围为 1.4 至 47 µg/mL，而原始纳米粘土的 IC50 值高于 100 µg/mL。有机粘土的 IC50 值取决于用作表面有机改性剂的季铵化合物的比例和结构。尽管纳米粘土批次的大小差异主要与粒径分布的上限有关，但大尺寸和小尺寸（额外的研磨步骤）纳米粘土批次之间未观察到细胞毒性差异。尽管原始纳米粘土的毒性较低，但它诱导了细胞凋亡，并在暴露细胞的细胞质小泡中被发现。有机粘土也存在于细胞质小泡中，尽管团聚体的尺寸更大，并且摄取效率要低得多。
