应用 IS630 分型对嗜水气单胞菌亚种进行聚类分析。

Clustering subspecies of Aeromonas salmonicida using IS630 typing.

机构信息

Institute of Veterinary Bacteriology, University of Bern, Länggassstrasse 122, Bern, Switzerland.

出版信息

BMC Microbiol. 2013 Feb 13;13:36. doi: 10.1186/1471-2180-13-36.

BACKGROUND

The insertion element IS630 found in Aeromonas salmonicida belongs to the IS630-Tc1-mariner superfamily of transposons. It is present in multiple copies and represents approximately half of the IS present in the genome of A. salmonicida subsp. salmonicida A449.

RESULTS

By using High Copy Number IS630 Restriction Fragment Length Polymorphism (HCN-IS630-RFLP), strains of various subspecies of Aeromonas salmonicida showed conserved or clustering patterns, thus allowing their differentiation from each other. Fingerprints of A. salmonicida subsp. salmonicida showed the highest homogeneity while 'atypical' A. salmonicida strains were more heterogeneous. IS630 typing also differentiated A. salmonicida from other Aeromonas species. The copy number of IS630 in Aeromonas salmonicida ranges from 8 to 35 and is much lower in other Aeromonas species.

CONCLUSIONS

HCN-IS630-RFLP is a powerful tool for subtyping of A. salmonicida. The high stability of IS630 insertions in A. salmonicida subsp. salmonicida indicates that it might have played a role in pathoadaptation of A. salmonicida which has reached an optimal configuration in the highly virulent and specific fish pathogen A. salmonicida subsp. salmonicida.

背景

嗜水气单胞菌中的插入元件 IS630 属于 IS630-Tc1-mariner 转座子超家族。它以多个拷贝的形式存在，大约占嗜水气单胞菌亚种 salmonicida A449 基因组中存在的 IS 的一半。

结果

通过使用高拷贝数 IS630 限制片段长度多态性（HCN-IS630-RFLP），不同亚种的嗜水气单胞菌菌株表现出保守或聚类模式，从而能够相互区分。嗜水气单胞菌亚种 salmonicida 的指纹图谱显示出最高的同质性，而“非典型”嗜水气单胞菌菌株则具有更高的异质性。IS630 分型还可将嗜水气单胞菌与其他气单胞菌物种区分开来。嗜水气单胞菌中的 IS630 拷贝数范围为 8 到 35，而在其他气单胞菌物种中则要低得多。