Ondokuz Mayis University, Vocational High School of Bafra, Samsun, Turkey.
Pharm Biol. 2013 Mar;51(3):391-9. doi: 10.3109/13880209.2012.733012.
The genus Hypericum (Guttiferae) has received considerable scientific interest as a source of biologically active compounds.
The study determined the morphogenetic and ontogenetic variation in the main bioactive compounds of two Hypericum species, namely, Hypericum aviculariifolium subsp. depilatum var. depilatum (Freyn and Bornm.) Robson var. depilatum and Hypericum orientale L. through HPLC analyses of whole plants as well as individual plant parts (stems, leaves, and reproductive tissues).
The plant materials were harvested at five phenological stages: vegetative, floral budding, full flowering, fresh fruiting, and mature fruiting; dried at room temperature, then assayed for chemical content.
In H. aviculariifolium, no kaempferol accumulation was observed and the highest level of hypericin, pseudohypericin, and quercitrin was reached at full flowering (0.71, 1.78, and 4.15 mg/g DW, respectively). Plants, harvested at floral budding produced the highest amount of rutin, hyperoside, and isoquercitrine (32.96, 2.42, 1.52 mg/g DW, respectively). H. orientale did not produce hypericin, pseudohypericin, or kaempferol. Rutin, hyperoside, and isoquercetine levels were the highest at floral development (1.76, 11.85, and 1.21 mg/g DW, respectively) and plants harvested at fresh fruiting produced the highest amount of quercitrine and quercetine (0.20 and 1.30 mg/g DW, respectively).
For the first time, the chemical composition of the Turkish species of Hypericum was monitored during the course of ontogenesis to determine the ontogenetic and morphogenetic changes in chemical content.
Plant material should be harvested during flower ontogenesis for medicinal purposes in which the content of many bioactive substances tested reached their highest level.
藤黄科贯叶金丝桃属植物因其含有生物活性化合物而备受关注。
本研究通过高效液相色谱法（HPLC）分析贯叶金丝桃（Hypericum aviculariifolium subsp. depilatum var. depilatum（Freyn and Bornm.）Robson var. depilatum）和东方贯叶金丝桃（Hypericum orientale L.）全株及各器官（茎、叶和生殖组织）中主要生物活性化合物的形态发生和个体发育变化。
在 5 个物候期（营养期、花蕾期、盛花期、鲜果期和成熟果期）采集植物材料；在室温下干燥，然后测定化学含量。
在 H. aviculariifolium 中未检测到山奈酚的积累，而金丝桃素、伪金丝桃素和槲皮苷的含量在盛花期达到最高水平（分别为 0.71、1.78 和 4.15mg/g DW）。在花蕾期收获的植物中，芦丁、金丝桃苷和异槲皮苷的含量最高（分别为 32.96、2.42 和 1.52mg/g DW）。H. orientale 不产生金丝桃素、伪金丝桃素或山奈酚。在花发育阶段，芦丁、金丝桃苷和异槲皮苷的含量最高（分别为 1.76、11.85 和 1.21mg/g DW），在新鲜果实期收获的植物中，槲皮苷和槲皮素的含量最高（分别为 0.20 和 1.30mg/g DW）。
首次监测了土耳其贯叶金丝桃属植物在个体发育过程中的化学成分，以确定化学含量的个体发育和形态发生变化。
为了药用目的，应在花发育阶段收获植物材料，因为测试的许多生物活性物质的含量达到最高水平。
