Tsumano Tomoko, Kawai Kenichiro, Ishise Hisako, Nishimoto Soh, Fukuda Kenji, Fujiwara Toshihiro, Kakibuchi Masao
Department of Plastic Surgery, Hyogo College of Medicine, Hyogo, Japan.
J Plast Surg Hand Surg. 2013 Apr;47(2):83-8. doi: 10.3109/2000656X.2012.731000. Epub 2013 Feb 14.
Radiation has many benefits and is an important treatment for cancer therapy. However, it also has unfavourable side-effects. Among these side-effects, the impairment of wound healing in the skin is a major problem in clinics. Although many attempts have been made to overcome this shortcoming, there are few effective treatments for impaired wound healing after irradiation. One reason for this is that it is hard to obtain good animal models for researching this topic. In this study, two different models were created and investigated. In one model, rectangular flaps were created on the backs of mice and irradiated while the other parts of their bodies were covered with a lead board. In another model, the lower limbs were exposed to radiation. In each model, several doses of irradiation were tested. Skin ulcers were created in the irradiated area, and the wound healing process was observed. In order to verify the usefulness of the model, adipose derived stromal cells were injected into the wound and the healing rate was calculated. In the flap model, the flaps contracted and formed linear scars. On the other hand, in the thigh model, 15 Gy irradiation resulted in slow wound healing but no strong inflammation or necrosis. The transplantation of adipose tissue derived stromal cells into the irradiated thigh wound improved the wound healing. This study suggested that irradiation of the lower limb at ∼ 15 Gy might be an appropriate model for basic research into wound healing in irradiated skin.
辐射有诸多益处，是癌症治疗的重要手段。然而，它也存在不良副作用。在这些副作用中，皮肤伤口愈合受损是临床上的一个主要问题。尽管人们已多次尝试克服这一缺点，但针对放疗后伤口愈合受损的有效治疗方法却寥寥无几。原因之一是很难获得用于研究该课题的良好动物模型。在本研究中，创建并研究了两种不同的模型。在一种模型中，在小鼠背部制作矩形皮瓣并进行照射，同时其身体的其他部位用铅板覆盖。在另一种模型中，下肢接受辐射。在每个模型中，测试了几种辐射剂量。在照射区域制造皮肤溃疡，并观察伤口愈合过程。为了验证该模型的实用性，将脂肪来源的基质细胞注入伤口并计算愈合率。在皮瓣模型中，皮瓣收缩并形成线性瘢痕。另一方面，在大腿模型中，15 Gy的辐射导致伤口愈合缓慢，但没有强烈的炎症或坏死。将脂肪组织来源的基质细胞移植到受照射的大腿伤口可改善伤口愈合。本研究表明，约15 Gy的下肢照射可能是用于照射皮肤伤口愈合基础研究的合适模型。
