Aksoy Laçine
Biochemistry Division, Department of Chemistry, Faculty of Science and Arts, Afyon Kocatepe University, Afyonkarahisar, Turkey
Toxicol Ind Health. 2015 May;31(5):442-7. doi: 10.1177/0748233713475513. Epub 2013 Feb 13.
This study was conducted to compare the effects of oral toxicity induced by fish oil biodiesel and diesel fuel. Diesel and fish oil biodiesel were administered by oral gavage to rats. For this purpose, 35 rats were divided into five groups. Sunflower oil of 250 mg kg(-1) was administered to the rats in the control group by oral gavage. The rats in the D250 and D500 groups were administered by oral gavage 250 mg kg(-1) and 500 mg kg(-1) of diesel fuel dissolved in equal amounts of sunflower oil, respectively. The rats in the F250 and F500 groups were administered by oral gavage 250 mg kg(-1) and 500 mg kg(-1) of fish oil biodiesel dissolved in equal amounts of sunflower oil, respectively. At the end of the study, malondialdehyde (MDA) and reduced glutathione (GSH) levels were measured in the whole blood; catalase (CAT) activity level was measured in erythrocytes; and nitrite (NO2) and nitrate (NO3) levels were measured in the serum. It was observed that the whole blood MDA levels of the diesel groups were considerably different from those in the control and fish oil biodiesel groups (p < 0.001). GSH levels in the control group were observed to be considerably different from those in all other groups (p < 0.001). Serum NO3 concentrations in the diesel groups were found to be considerably different from those in the control and biodiesel groups. Serum NO2 concentrations in one of the diesel groups were significantly different from those in the control and biodiesel groups (p < 0.01 and p < 0.05, respectively). The CAT activity of the control group was observed to be different from that in the other groups. According to these results, both fish oil biodiesel and diesel fuel are thought to cause lipid peroxidation. It was observed that fish oil biodiesel does not induce as much oxidative damage as does the diesel fuel. It is suggested that fish oil biodiesel should be preferred as an alternative to the diesel.
本研究旨在比较鱼油生物柴油和柴油引起的口服毒性效应。通过口服灌胃法给大鼠施用柴油和鱼油生物柴油。为此，将35只大鼠分为五组。对照组大鼠通过口服灌胃给予250 mg kg(-1)的向日葵油。D250组和D500组大鼠分别通过口服灌胃给予溶解于等量向日葵油中的250 mg kg(-1)和500 mg kg(-1)柴油。F250组和F500组大鼠分别通过口服灌胃给予溶解于等量向日葵油中的250 mg kg(-1)和500 mg kg(-1)鱼油生物柴油。在研究结束时，测定全血中的丙二醛（MDA）和还原型谷胱甘肽（GSH）水平；测定红细胞中的过氧化氢酶（CAT）活性水平；测定血清中的亚硝酸盐（NO2）和硝酸盐（NO3）水平。观察到柴油组的全血MDA水平与对照组和鱼油生物柴油组有显著差异（p < 0.001）。观察到对照组的GSH水平与所有其他组有显著差异（p < 0.001）。发现柴油组的血清NO3浓度与对照组和生物柴油组有显著差异。其中一个柴油组的血清NO2浓度与对照组和生物柴油组有显著差异（分别为p < 0.01和p < 0.05）。观察到对照组的CAT活性与其他组不同。根据这些结果，认为鱼油生物柴油和柴油燃料均会引起脂质过氧化。观察到鱼油生物柴油引起的氧化损伤不如柴油燃料严重。建议优先选择鱼油生物柴油作为柴油的替代品。
