Zusman S, Patel-King R S, Ffrench-Constant C, Hynes R O
Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139.
Development. 1990 Mar;108(3):391-402. doi: 10.1242/dev.108.3.391.
The common beta subunit of the PS antigens of Drosophila is homologous with vertebrate integrins and is encoded by the lethal(1)myospheroid gene. We have generated flies mosaic for wild-type and mutant alleles of lethal(1)myospheroid using adult gynandromorphs and radiation-induced somatic crossing over. The defects observed in the gynandromorphs demonstrate widespread requirements for PS integrins during development especially in ventrally derived structures, which also show strong expression of PS beta integrin. Smaller lethal(1)myospheroid clones induced during larval development result in blister and vein defects in the wings and aberrant development of photoreceptor cells, demonstrating roles for PS integrins during development of both wings and eyes. PS integrins are required for the close apposition of the dorsal and ventral wing epithelia and for the proper arrangement of photoreceptor cells. However, many other adhesive and morphogenetic processes proceed normally in the absence of integrins containing the beta subunit encoded by lethal(1)myospheroid.
果蝇PS抗原的共同β亚基与脊椎动物整合素同源，由致死（1）肌球样基因编码。我们利用成年雌雄嵌合体和辐射诱导的体细胞交换，产生了致死（1）肌球样野生型和突变等位基因的嵌合果蝇。在雌雄嵌合体中观察到的缺陷表明，PS整合素在发育过程中广泛存在需求，特别是在腹侧衍生结构中，这些结构也强烈表达PSβ整合素。幼虫发育期间诱导产生的较小的致死（1）肌球样克隆会导致翅膀出现水泡和静脉缺陷以及光感受器细胞发育异常，这表明PS整合素在翅膀和眼睛发育过程中发挥作用。PS整合素是背侧和腹侧翅膀上皮紧密贴合以及光感受器细胞正确排列所必需的。然而，在缺乏由致死（1）肌球样编码的β亚基的整合素的情况下，许多其他黏附及形态发生过程仍正常进行。
